Cytokine gene expression of peripheral blood lymphocytes stimulated by lipopolysaccharide
Cytokine gene expression of peripheral blood lymphocytes stimulated by lipopolysaccharide
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脂多糖刺激外周血淋巴细胞细胞因子基因表达
作者：
脂多糖（LPS）是γ阴性细菌外膜中的主要糖脂，可刺激单核细胞、巨噬细胞和中性粒细胞产生细胞因子。目的是研究TLRs和细胞因子的表达谱，并确定LPS在外周血淋巴细胞中的作用。从鸡外周血中分离淋巴细胞，用LPS诱导，提取总RNA并逆转录为cDNA。采用半定量RT-PCR定量检测TLRs和炎症因子mRNA表达水平。结果显示，淋巴细胞表达TLRs （TLR1/6/10、TLR3、TLR4、TLR5、TLR7）和白细胞介素（IL-1、IL-8、IL-18、TGF- 4） mRNA。LPS显著（P 0.05）诱导TLR1/6/10、TLR5和TGF- 4 mRNA表达。脂多糖在淋巴细胞对病原体的先天免疫反应中起重要作用。
Lipopolysaccharide (LPS) is a predominant glycolipid in the outer membranes of gam-negative bacteria that stimulates monocytes, macrophages, and neutrophils to produce cytokines. The aim was to study the expression profile of TLRs and cytokines and determine the role of LPS in the peripheral blood lymphocytes. Lymphocytes were isolated from the peripheral blood of chickens and induced with LPS, total RNA was extracted and reverse transcribed into cDNA. The mRNA expression level of TLRs and inflammatory cytokines were quantitatively determined by semi-quantitative RT-PCR. The result showed that lymphocytes were expressed mRNA of TLRs (TLR1/6/10, TLR3, TLR4, TLR5 and TLR7) and interleukins (IL-1�, IL-8, IL-18 and TGF-�4). LPS will significantly (P 0.05) induce the mRNA expression of TLR1/6/10, TLR5 and TGF-�4. LPS plays an important role in the innate immune response of lymphocytes to pathogen.
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影响因子：
2.1
作者：
H. Robinson
通讯作者：
H. Robinson
DOI：
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发表时间：
2005
期刊：
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
影响因子：
--
作者：
M. Kogut;L. Rothwell;P. Kaiser
通讯作者：
M. Kogut;L. Rothwell;P. Kaiser
影响因子：
1.8
作者：
Iqbal, M;Philbin, VJ;Smith, AL
通讯作者：
Smith, AL
影响因子：
4.4
作者：
V. Verhasselt;C. Buelens;F. Willems;D. Groote;N. Haeffner‐cavaillon;M. Goldman
通讯作者：
V. Verhasselt;C. Buelens;F. Willems;D. Groote;N. Haeffner‐cavaillon;M. Goldman
影响因子：
29.7
作者：
K. Takeda;T. Kaisho;S. Akira
通讯作者：
K. Takeda;T. Kaisho;S. Akira