Proteomics shed light on the brassinosteroid signaling mechanisms.

Proteomics shed light on the brassinosteroid signaling mechanisms.
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DOI:
10.1016/j.pbi.2009.10.007
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发表时间:
2010-02
影响因子:
9.5
通讯作者:
Wang ZY
Wang ZY
中科院分区:
生物学2区
文献类型:
--
作者:
Tang W;Deng Z;Wang ZY

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大量受体样激酶(RLK)通过感知细胞外信号在植物发育和防御中发挥关键作用。配体诱导的激酶激活和下游信号转导的机制仅针对少数 RLK 途径进行了研究,其中油菜素类固醇 (BR) 途径的特征最为明确。最近,蛋白质组学研究发现了新的成分,可以弥补基因定义的 BR 信号通路中最后一个缺口,建立植物中从 RLK 到转录因子的第一个完整通路。此外,对磷酸化事件的分析(主要通过质谱法)提供了对受体激酶激活和磷酸化下游成分调节的机制细节的见解。本综述重点介绍蛋白质组学研究在理解 BR 信号转导方面的最新进展。
Large numbers of receptor-like kinases (RLKs) play key roles in plant development and defense by perceiving extracellular signals. The mechanisms of ligand-induced kinase activation and downstream signal transduction have been studied for only a few RLK pathways, among which the brassinosteroid (BR) pathway is the best characterized. Recently, proteomics studies identified new components that bridge the last gap in the genetically defined BR-signaling pathway, establishing the first complete pathway from an RLK to transcription factors in plants. Furthermore, analyses of phosphorylation events, mostly by mass spectrometry, provided insights into the mechanistic details of receptor kinase activation and regulation of downstream components by phosphorylation. This review focuses on recent progress in understanding BR signal transduction made by proteomics studies.
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