A clostripain-like protease plays a major role in generating the secretome of enterotoxigenic Bacteroides fragilis.
A clostripain-like protease plays a major role in generating the secretome of enterotoxigenic Bacteroides fragilis.
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一种梭状蛋白酶在产生产肠毒素的脆弱拟杆菌的分泌组中起主要作用。
DOI：
10.1111/mmi.14616
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发表时间：
2021-03
影响因子：
3.6
通讯作者：
Bernstein, Harris D.
中科院分区：
文献类型：
--
作者：
Pierce, Jessica V.;Fellows, Justin D.;Anderson, D. Eric;Bernstein, Harris D.
脆弱拟杆菌毒素（BFT）是脆弱杆菌产肠毒素（ETBF）菌株分泌的一种蛋白质。BFT被合成为一种蛋白质（proBFT），预计是一种脂蛋白，它被一种叫做fragipain （Fpn）的类似clostripain的蛋白酶切割成两个离散的片段。在这项研究中，我们获得了Fpn在其通过外膜运输后分裂proBFT的证据。值得注意的是，我们还发现fpn基因的破坏导致ETBF菌株培养基中bbb100其他蛋白质水平的强烈降低，其中许多被预测为脂蛋白。用纯化的Fpn进行的实验提供了直接证据，证明蛋白酶至少从细胞表面释放了这些蛋白质中的一些。在共培养实验中，野生型细胞胜过fpn -菌株的观察结果也支持了fpn在细胞生理学中起重要作用的观点，而不仅仅是致力于毒素的生物生成。最后，我们发现纯化的Fpn改变了HT29肠上皮细胞的粘附特性。我们的研究结果表明，Fpn是一种广谱蛋白酶，不仅在大范围内催化蛋白质分泌，而且在定植过程中也可能切割宿主细胞蛋白质。
Bacteroides fragilis toxin (BFT) is a protein secreted by enterotoxigenic (ETBF) strains of B. fragilis. BFT is synthesized as a proprotein (proBFT) that is predicted to be a lipoprotein and that is cleaved into two discrete fragments by a clostripain-like protease called fragipain (Fpn). In this study, we obtained evidence that Fpn cleaves proBFT following its transport across the outer membrane. Remarkably, we also found that the disruption of the fpn gene led to a strong reduction in the level of >100 other proteins, many of which are predicted to be lipoproteins, in the culture medium of an ETBF strain. Experiments performed with purified Fpn provided direct evidence that the protease releases at least some of these proteins from the cell surface. The observation that wild-type cells outcompeted an fpn− strain in co-cultivation assays also supported the notion that Fpn plays an important role in cell physiology and is not simply dedicated to toxin biogenesis. Finally, we found that purified Fpn altered the adhesive properties of HT29 intestinal epithelial cells. Our results suggest that Fpn is a broad-spectrum protease that not only catalyzes the protein secretion on a wide scale but that also potentially cleaves host cell proteins during colonization.
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肠道共生型细菌的抗菌蛋白具有宿主免疫蛋白的MACPF结构域。
影响因子：
3.6
作者：
Chatzidaki-Livanis M;Coyne MJ;Comstock LE
通讯作者：
Comstock LE
DOI：
10.1007/s00018-016-2242-x
发表时间：
2016-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Foley MH;Cockburn DW;Koropatkin NM
通讯作者：
Koropatkin NM
影响因子：
18.6
作者：
Guo Y;Nguyen KA;Potempa J
通讯作者：
Potempa J
影响因子：
3.1
作者：
Franco, AA;Buckwold, SL;Sears, CL
通讯作者：
Sears, CL
影响因子：
3.1
作者：
Ichimura, Minoru;Uchida, Keiko;Kuwahara, Tomomi
通讯作者：
Kuwahara, Tomomi