Gastruloids - a minimalistic model to study complex developmental metabolism.
Gastruloids - a minimalistic model to study complex developmental metabolism.
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DOI：
10.1042/etls20230082
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发表时间：
2023-12-18
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
代谢网络能够很好地协调与胚胎发育相关的多个细胞过程，例如细胞生长、增殖、分化和细胞运动。在这里，我们讨论原肠胚（自组装基本身体计划的哺乳动物胚胎干细胞聚集体）的优势，以揭示代谢途径在指导发育过程中发挥的指导作用。我们强调使用这种还原论系统将特定途径与早期哺乳动物发育的确定事件联系起来的重要性及其为代谢组学研究获得足够材料的效用。最后，我们回顾了如何调整基本原肠胚协议以获得胚胎细胞类型、组织和区域的特定模型。总之，我们认为原肠胚是一个理想的系统，可以快速揭示发育信号通路和代谢网络之间的新机制联系，然后可以为精确的体内研究提供信息，以确认它们在胚胎中的功能。
Metabolic networks are well placed to orchestrate the coordination of multiple cellular processes associated with embryonic development such as cell growth, proliferation, differentiation and cell movement. Here, we discuss the advantages that gastruloids, aggregates of mammalian embryonic stem cells that self-assemble a rudimentary body plan, have for uncovering the instructive role of metabolic pathways play in directing developmental processes. We emphasise the importance of using such reductionist systems to link specific pathways to defined events of early mammalian development and their utility for obtaining enough material for metabolomic studies. Finally, we review the ways in which the basic gastruloid protocol can be adapted to obtain specific models of embryonic cell types, tissues and regions. Together, we propose that gastruloids are an ideal system to rapidly uncover new mechanistic links between developmental signalling pathways and metabolic networks, which can then inform precise in vivo studies to confirm their function in the embryo.
影响因子：
2.7
作者：
Busby L;Saunders D;Nájera GS;Steventon B
通讯作者：
Steventon B
影响因子：
2.9
作者：
Patel, Jeet H.;Wills, Andrea E.
通讯作者：
Wills, Andrea E.