Temporal progression of the host response to implanted poly(ethylene glycol)-based hydrogels.
Temporal progression of the host response to implanted poly(ethylene glycol)-based hydrogels.
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DOI：
10.1002/jbm.a.33015
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发表时间：
2011-03-15
影响因子：
4.9
通讯作者：
Bryant, Stephanie J.
中科院分区：
文献类型：
--
作者：
Lynn, Aaron D.;Blakney, Anna K.;Kyriakides, Themis R.;Bryant, Stephanie J.
聚乙二醇（PEG）水凝胶作为组织工程的体内细胞载体具有广阔的前景。为了确保这些材料在植入时具有适当的性能，必须充分了解宿主的反应。本研究的目的是通过（免疫）组织化学分析和基因表达来表征当将由 PEG 二丙烯酸酯前体制备的无细胞 PEG 水凝胶植入免疫功能正常的 c57bl/6 小鼠皮下时，异物反应 (FBR) 的时间演变。与硅酮 (SIL) 引发的正常 FBR 相比，不含或含细胞粘附配体 RGD 的 PEG 水凝胶可引发强烈的早期炎症反应，这一点可从第 2 天出现一条厚巨噬细胞带并持续长达 2 周以及白细胞介素 1β 表达增加来证明。与 PEG-RGD 相比，纯 PEG 水凝胶显示出更慢但更持续的炎症进展。观察到对基于 PEG 的材料的基因表达的时间变化，并且通常表现出植入物周围组织中炎症和伤口愈合基因的表达升高，而对 SIL 的反应表达模式更加稳定。虽然使用 SIL 实现了稳定的 FBR，并且使用 PEG-RGD 实现了较小程度的稳定，但仅 PEG 的水凝胶在 4 周后尚未稳定。总之，纯 PEG 水凝胶会引发强烈的早期炎症反应，这种反应在整个植入过程中持续存在，甚至在胶原胶囊开始形成时也是如此。然而，RGD 系链的掺入在 2 周内部分减弱了这种反应，从而改善了基于 PEG 的水凝胶的 FBR。
Poly(ethylene glycol) (PEG) hydrogels hold great promise as in vivo cell carriers for tissue engineering. To ensure appropriate performance of these materials when implanted, the host response must be well understood. The objectives for this study were to characterize the temporal evolution of the foreign body reaction (FBR) to acellular PEG-based hydrogels prepared from PEG diacrylate precursors when implanted subcutaneously in immunocompentent c57bl/6 mice by (immuno)histochemical analysis and gene expression. Compared to a normal FBR elicited by silicone (SIL), PEG hydrogels without or with a cell adhesion ligand RGD elicited a strong early inflammatory response evidenced by a thick band of macrophages as early as day 2, persisting through 2 weeks, and by increased interleukin-1β expression. PEG-only hydrogels showed a slower, but more sustained progression of inflammation over PEG-RGD. Temporal changes in gene expression were observed in response to PEG-based materials and in general exhibited, elevated expression of inflammatory and wound healing genes in the tissues surrounding the implants, while the expression patterns were more stable in response to SIL. While a stabilized FBR was achieved with SIL and to a lesser degree with PEG-RGD, the PEG-only hydrogels had not yet stabilized after 4 weeks. In summary, PEG-only hydrogels elicit a strong early inflammatory reaction, which persists throughout the course of the implantation even as a collagenous capsule begins to form. However, the incorporation of RGD tethers partially attenuates this response within 2 weeks leading to an improved FBR to PEG-based hydrogels.
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影响因子：
14
作者：
Chen, Sulin;Jones, Jacqueline A.;Xu, Yongan;Low, Hong-Yee;Anderson, James M.;Leong, Kam W.
通讯作者：
Leong, Kam W.
DOI：
10.1302/0301-620x.79b3.7469
发表时间：
1997-05-01
影响因子：
--
作者：
Hukkanen, M;Corbett, SA;Polak, JM
通讯作者：
Polak, JM
影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
影响因子：
3.3
作者：
Cruise, GM;Hegre, OD;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
4.9
作者：
Iyer, Rohin K.;Chiu, Loraine L. Y.;Radisic, Milica
通讯作者：
Radisic, Milica