Transient activation of Wnt/β-catenin signaling reporter in fibrotic scar formation after compression spinal cord injury in adult mice.
Transient activation of Wnt/β-catenin signaling reporter in fibrotic scar formation after compression spinal cord injury in adult mice.
复制标题
DOI：
10.1016/j.bbrc.2018.02.004
复制
发表时间：
2018-02-19
影响因子：
3.1
通讯作者：
Zhou CJ
中科院分区：
文献类型：
--
作者：
Yamagami T;Pleasure DE;Lam KS;Zhou CJ
创伤性脊髓损伤（SCI）后，可能会在损伤部位形成具有纤维化核心（纤维化瘢痕）和周围反应性星形胶质细胞（神经胶质瘢痕）的瘢痕。瘢痕组织被认为是阻止再生的主要障碍，既作为物理屏障又作为轴突再生抑制剂的分泌来源。了解疤痕形成的机制以及如何控制它可能会导致有效的SCI治疗。使用成年转基因小鼠的压缩-SCI模型，我们证明了典型的Wnt/β-catenin信号报告基因TOPgal（TCF/Lef 1-lacZ）阳性细胞在损伤部位出现5天，在7天达到峰值，并在损伤后14天减少。使用各种代表性的细胞谱系标记物，我们证明，这些短暂的TOPgal阳性细胞是一组纤连蛋白（+）;GFAP（-）成纤维细胞样细胞在核心瘢痕区域。其中一些是增殖性的。这些结果表明Wnt/β-catenin信号通路可能在创伤性脊髓损伤后纤维化瘢痕形成中起关键作用。
After traumatic spinal cord injury (SCI), a scar may form with a fibrotic core (fibrotic scar) and surrounding reactive astrocytes (glial scar) at the lesion site. The scar tissue is considered a major obstacle preventing regeneration both as a physical barrier and as a source for secretion of inhibitors of axonal regeneration. Understanding the mechanism of scar formation and how to control it may lead to effective SCI therapies. Using a compression-SCI model on adult transgenic mice, we demonstrate that the canonical Wnt/β-catenin signaling reporter TOPgal (TCF/Lef1-lacZ) positive cells appeared at the lesion site by 5 days, peaked on 7 days, and diminished by 14 days post injury. Using various representative cell lineage markers, we demonstrate that, these transiently TOPgal positive cells are a group of Fibronectin(+);GFAP(−) fibroblast-like cells in the core scar region. Some of them are proliferative. These results indicate that Wnt/β-catenin signaling may play a key role in fibrotic scar formation after traumatic spinal cord injury.
登录
查看更多内容
影响因子：
5.3
作者：
Popovich, Phillip G.;Tovar, C. Amy;Wei, Ping;Fisher, Lesley;Jakeman, Lyn B.;Basso, D. Michele
通讯作者：
Basso, D. Michele
影响因子：
4.8
作者：
Bondjers, Cecilia;He, Liqun;Betsholtz, Christer
通讯作者：
Betsholtz, Christer
影响因子：
3
作者：
Poon, Peter C.;Gupta, Dimpy;Tator, Charles H.
通讯作者：
Tator, Charles H.
影响因子：
4.8
作者：
Hollis ER 2nd;Zou Y
通讯作者：
Zou Y
影响因子：
3.9
作者：
Bandtlow, CE
通讯作者：
Bandtlow, CE