Bioassay of cadmium using a DNA microarray: Genome‐wide expression patterns of Saccharomyces cerevisiae response to cadmium-猫眼课题宝

使用 DNA 微阵列进行镉的生物测定：酿酒酵母对镉响应的全基因组表达模式

DOI：

10.1002/etc.5620201030

发表时间：

2001

期刊：

Environmental Toxicology and Chemistry

影响因子：

4.1

通讯作者：

中科院分区：

环境科学与生态学3区

文献类型：

作者：

Y. Momose;H. Iwahashi

DNA微阵列技术能够在转录水平上对全基因组范围的细胞反应进行检测。我们计划制造能够检测环境化学物质的生物检测系统，以筛选潜在的生物活性物质。为了建立适合我们目的的DNA微阵列，我们用总mRNA芯片分析了酵母对镉胁迫反应的基因表达变化。镉是一种强有力的细胞毒物，已知通过改变细胞内谷胱甘肽水平而导致氧化应激。本文报道了镉暴露后，不仅谷胱甘肽合成基因(GSH1)，而且几乎所有与硫氨基酸代谢有关的酶的转录产物，特别是MET14和MET17，都被大量诱导。虽然几个常见的应激反应基因，如HSP26、GRE1、HSP12和DDR48，在镉的作用下上调了近四倍以上，但也有42个基因上调了四倍以上。基于这些结果，我们得出结论，DNA微阵列是创建新的生物检测系统和寻找压力指标的遗传启动子的非常有用的工具。

DNA microarray technology enables genome‐wide detection of cell response at the transcriptional level. We are planning to make bioassay systems that can detect environmental chemicals to screen for potential bioreactive agents. To develop a DNA microarray for our purposes, the changes in gene expression underlying the yeast stress response to cadmium were analyzed by a microarray of total mRNA. Cadmium is a potent cell poison known to cause oxidative stress by changing intracellular glutathione levels. We report here that not only the glutathione synthesis gene (GSH1) but also almost all transcripts of the enzymes involved in the sulfur amino acid metabolism, especially MET14 and MET17, were greatly induced after exposure to cadmium. While several common stress‐responsive genes, such as HSP26, GRE1, HSP12, and DDR48, were up‐regulated more than almost fourfold by cadmium, there were also 42 other genes up‐regulated more than fourfold. Based on these results, we concluded that DNA microarrays are very useful instruments for creating new bioassay systems and finding genetic promoters of stress indicators.