Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny.
Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny.
复制标题
DOI：
10.1186/s12983-016-0177-6
复制
发表时间：
2016
影响因子：
2.8
通讯作者：
Sordino P
中科院分区：
文献类型：
--
作者：
Valero-Gracia A;Marino R;Crocetta F;Nittoli V;Tiozzo S;Sordino P
海樽纲是鳖门中研究较少的纲之一。特别是，它们的系统发育关系仍然是一个有争议的问题。5-羟色胺（5-HT）分布的整体模式是解释内部关系的一个很好的生化特征。在这里报告的实验中，我们比较了两种脂质体，一种脂质体和一种焦虫体在不同生命周期阶段的血清素样免疫反应性。这种多物种的比较为更好地解决海棠纲的系统发育问题提供了新的神经解剖学数据。所有四种被检测的海thalacean的成年物种在神经元和非神经元细胞类型中表现出5 -羟色胺样免疫反应性，其神经系统的解剖位置由于α-微管蛋白免疫反应性而一致可识别。结果表明，与血清素在不同形态和位置的细胞体中的存在一致的广泛模式，在目内和目间有一些变化。在囊虫（Salpida）、蝌蚪幼虫（oliolida）和幼虫（Pyrosomatida）等未成熟动物中未发现血清素样免疫反应性。在所有三种海藻类分支中，5 -羟色胺样免疫反应性的比较解剖学先前尚未报道。这些结果讨论了有关研究的5 -羟色胺样免疫反应在成年海鞘。Salpida和ololida与Pyrosomella verticillata相比，内层缺乏5 -羟色胺样免疫反应性可能是5 -羟色胺对纤毛跳动和粘液分泌的控制继发性丧失的结果。这些数据，当与其他多形性特征相结合时，支持了火虫纲是这些分支的基础，而Salpida和Doliolida是姐妹类群的假设。本文的在线版本（doi:10.1186/s12983-016-0177-6）包含补充材料，可供授权用户使用。
Thaliaceans is one of the understudied classes of the phylum Tunicata. In particular, their phylogenetic relationships remain an issue of debate. The overall pattern of serotonin (5-HT) distribution is an excellent biochemical trait to interpret internal relationships at order level. In the experiments reported here we compared serotonin-like immunoreactivity at different life cycle stages of two salpid, one doliolid, and one pyrosomatid species. This multi-species comparison provides new neuroanatomical data for better resolving the phylogeny of the class Thaliacea. Adults of all four examined thaliacean species exhibited serotonin-like immunoreactivity in neuronal and non-neuronal cell types, whose anatomical position with respect to the nervous system is consistently identifiable due to α-tubulin immunoreactivity. The results indicate an extensive pattern that is consistent with the presence of serotonin in cell bodies of variable morphology and position, with some variation within and among orders. Serotonin-like immunoreactivity was not found in immature forms such as blastozooids (Salpida), tadpole larvae (Doliolida) and young zooids (Pyrosomatida). Comparative anatomy of serotonin-like immunoreactivity in all three thaliacean clades has not been reported previously. These results are discussed with regard to studies of serotonin-like immunoreactivity in adult ascidians. Lack of serotonin-like immunoreactivity in the endostyle of Salpida and Doliolida compared to Pyrosomella verticillata might be the result of secondary loss of serotonin control over ciliary beating and mucus secretion. These data, when combined with other plesiomorphic characters, support the hypothesis that Pyrosomatida is basal to these clades within Phlebobranchiata and that Salpida and Doliolida constitute sister-groups. The online version of this article (doi:10.1186/s12983-016-0177-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
1
作者：
Braun, Katrin;Stach, Thomas
通讯作者：
Stach, Thomas
影响因子：
2.6
作者：
Colas, JF;Launay, JM;Maroteaux, L
通讯作者：
Maroteaux, L
影响因子：
64.8
作者：
BARRINGTON, EJW;FRANCHI, LL
通讯作者：
FRANCHI, LL
DOI：
10.1016/0165-3806(85)90145-2
发表时间：
1985-01-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
EKSTROM, P;NYBERG, L;VANVEEN, T
通讯作者：
VANVEEN, T
影响因子：
2.8
作者：
Doran, SA;Koss, R;Goldberg, JI
通讯作者：
Goldberg, JI