Delayed re-epithelialization in periostin-deficient mice during cutaneous wound healing.
Delayed re-epithelialization in periostin-deficient mice during cutaneous wound healing.
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DOI：
10.1371/journal.pone.0018410
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发表时间：
2011-04-07
期刊：
影响因子：
3.7
通讯作者：
Kudo A
中科院分区：
文献类型：
--
作者：
Nishiyama T;Kii I;Kashima TG;Kikuchi Y;Ohazama A;Shimazaki M;Fukayama M;Kudo A
包括骨膜蛋白在内的基质细胞蛋白对组织再生很重要。目前，我们研究了骨膜蛋白在皮肤伤口愈合中的功能，使用骨膜蛋白缺陷（-/-）小鼠。Periostin mRNA在表皮和毛囊中均有表达，Periostin蛋白与纤维连接蛋白、层粘连蛋白γ2共同定位于毛囊基底膜。骨膜蛋白与层粘连蛋白γ2结合，这种结合增强了层粘连蛋白γ2长型的蛋白水解裂解，产生其短型。为了解决骨膜蛋白在伤口愈合中的作用，我们采用了使用WT和骨膜蛋白−/−小鼠的伤口愈合模型和体外划痕伤口测定。我们发现，骨膜蛋白−/−小鼠的伤口闭合延迟，再上皮化延迟，角质形成细胞增殖减少。此外，角质形成细胞增殖在骨膜蛋白过表达的HaCaT细胞中增强，沿着磷酸化NF-κB上调。这些结果表明，骨膜蛋白是必不可少的角质形成细胞增殖的再上皮化在皮肤伤口愈合。
Matricellular proteins, including periostin, are important for tissue regeneration. Presently we investigated the function of periostin in cutaneous wound healing by using periostin-deficient (−/−) mice. Periostin mRNA was expressed in both the epidermis and hair follicles, and periostin protein was located at the basement membrane in the hair follicles together with fibronectin and laminin γ2. Periostin was associated with laminin γ2, and this association enhanced the proteolytic cleavage of the laminin γ2 long form to produce its short form. To address the role of periostin in wound healing, we employed a wound healing model using WT and periostin−/− mice and the scratch wound assay in vitro. We found that the wound closure was delayed in the periostin−/− mice coupled with a delay in re-epithelialization and with reduced proliferation of keratinocytes. Furthermore, keratinocyte proliferation was enhanced in periostin-overexpressing HaCaT cells along with up-regulation of phosphorylated NF-κB. These results indicate that periostin was essential for keratinocyte proliferation for re-epithelialization during cutaneous wound healing.
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影响因子：
5.3
作者：
Nikolopoulos, SN;Blaikie, P;Giancotti, FG
通讯作者：
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影响因子：
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20.1
作者：
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通讯作者：
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