A robust protocol for regional evaluation of methacholine challenge in mouse models of allergic asthma using hyperpolarized 3He MRI.
A robust protocol for regional evaluation of methacholine challenge in mouse models of allergic asthma using hyperpolarized 3He MRI.
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DOI：
10.1002/nbm.1362
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发表时间：
2009-06
影响因子：
2.9
通讯作者：
Driehuys, Bastiaan
中科院分区：
文献类型：
--
作者：
Thomas, Abraham C.;Potts, Erin N.;Chen, Ben T.;Slipetz, Deborah M.;Foster, W. Michael;Driehuys, Bastiaan
超极化3 He磁共振成像（MRI）需要介绍术语，除非你超过了字数最近已被用于产生高分辨率图像的肺通气后乙酰甲胆碱激发小鼠模型的过敏性炎症。这种能力提供了一个机会，以获得有关这些模型的新见解，并在临床前环境中更敏感地评估新药治疗。在目前的研究中，我们提出了我们的初步经验，使用二维，时间分辨3 He磁共振成像乙酰甲胆碱挑战诱导的气道高反应性比较卵清蛋白致敏和挑战（N = 8）小鼠对照组（N =8）。成像表明，卵清蛋白致敏和挑战的动物表现出许多大的通气缺陷，甚至在乙酰甲胆碱挑战（4/8）相比，没有缺陷的对照组动物。此外，在乙酰甲胆碱输注后，卵清蛋白致敏和激发的动物经历了比对照组（3.3±0.6）更多的通气缺陷（4.5±0.4）。然而，由于小动物MRI环境特有的乙酰甲胆碱输送的变异性，平均缺损数量的差异不具有统计学显著性。这些研究结果进行了详细审查，并提出了一个全面的解决方案的变异性问题，大大提高了乙酰甲胆碱反应的幅度和重现性。这使我们能够开发一种新的成像方案，包括基线3D图像，乙酰甲胆碱激发期间的时间分辨2D系列，以及乙酰甲胆碱后3D图像，显示持续的通气缺陷。
Hyperpolarized 3He magnetic resonance imaging (MRI) NEED TO INTRODUCE TERM, UNLESS YOU ARE OVER THE WORD COUNThas been recently used to produce high-resolution images of pulmonary ventilation after methacholine challenge in mouse models of allergic inflammation. This capability presents an opportunity to gain new insights about these models and to more sensitively evaluate new drug treatments in the pre-clinical setting. In the current study, we present our initial experience using two-dimensional, time-resolved 3He MRI of methacholine challenge-induced airways hyperreactivity to compare ovalbumin-sensitized and challenged (N=8) mice to controls (N=8). Imaging demonstrated that ovalbumin-sensitized and challenged animals exhibited many large ventilation defects even prior to methacholine challenge (4 out of 8) compared to no defects in the control animals. Additionally, the ovalbumin sensitized and challenged animals experienced a greater number of ventilation defects (4.5±0.4) following methacholine infusion than did controls (3.3±0.6). However, due to variability in methacholine delivery that was specific to the small animal MRI environment, the difference in mean defect number was not statistically significant. These findings are reviewed in detail and a comprehensive solution to the variability problem is presented that has greatly enhanced the magnitude and reproducibility of the methacholine response. This has permitted us to develop a new imaging protocol consisting of a baseline 3D image, a time-resolved 2D series during methacholine challenge, and a post-methacholine 3D image that reveals persistent ventilation defects.
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DOI：
10.1016/s0731-7085(01)00377-6
发表时间：
2001-07-01
影响因子：
3.4
作者：
Acar, V;Houri, JJ;Guyon, F
通讯作者：
Guyon, F
DOI：
10.1152/ajpregu.1999.276.4.r1214
发表时间：
1999-04-01
影响因子：
2.8
作者：
Walker, JKL;Peppel, K;Fisher, JT
通讯作者：
Fisher, JT
影响因子：
9.6
作者：
de lange, Eduard E.;Altes, Talissa A.;Platts-Mills, Thomas A.
通讯作者：
Platts-Mills, Thomas A.
影响因子：
4.4
作者：
Altes, TA;Powers, PL;Brookeman, JR
通讯作者：
Brookeman, JR
影响因子：
3.3
作者：
Chen, BT;Yordanov, AT;Johnson, GA
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Johnson, GA