Leukocyte telomere dynamics in the elderly.
Leukocyte telomere dynamics in the elderly.
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老年人的白细胞端粒动力学。
DOI：
10.1007/s10654-013-9780-4
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发表时间：
2013-02
影响因子：
13.6
通讯作者：
Aviv, Abraham
中科院分区：
文献类型：
--
作者：
Steenstrup, Troels;Hjelmborg, Jacob V. B.;Mortensen, Laust H.;Kimura, Masayuki;Christensen, Kaare;Aviv, Abraham
有限的数据表明，老年人的白细胞显示超短端粒。有研究表明，老年人白细胞端粒长度（LTL）随年龄增长而延长。使用横截面和纵向模型，我们的特点是LTL动态参与者的纵向研究老化丹麦双胞胎。我们在横断面评估中通过末端限制性片段长度（TRFL）的Southern印迹法测量了476名个体（73-94岁）的LTL，并在该队列的一个子集中包括80名个体（基线时73-81岁），随访约10年。根据平均TRFL，我们发现a）横向和纵向评估的平均LTL损耗率分别为27 bp/年（P < 0.001）和31 bp/年（P < 0.001），B）女性的平均TRFL比男性长180 bp（95%CI 43，320）（P < 0.010）。对于TRFL分布，它捕获了DNA样本中所有长度的端粒，我们观察到随着年龄的增长，端粒向更短的方向发生了显着变化。基于TRFL的测量误差，我们计算出在纵向评估中，10.6%的个体将在10年内表现出LTL延长，假设在此期间有340 bp的损耗。这与7.5%的个体表现出LTL延长的经验观察没有显著差异。我们的结论是，老年人白细胞中的超短端粒的积累反映了整个端粒分布向较短端粒的转变。测量误差是纵向研究中LTL延长的可能原因。本文的在线版本（doi：10.1007/s10654-013-9780-4）包含补充材料，可供授权用户使用。
Limited data suggest that leukocytes of the elderly display ultra-short telomeres. It was reported that in some elderly persons leukocyte telomere length (LTL) shows age-dependent elongation. Using cross-sectional and longitudinal models, we characterized LTL dynamics in participants of the Longitudinal Study of Aging Danish Twins. We measured LTL by Southern blots of the terminal restriction fragment length (TRFL) in 476 individuals (73–94 years) in a cross-sectional evaluation and in a subset of this cohort comprising 80 individuals (73–81 years at baseline) who were followed–up for approximately 10 years. Based on the mean TRFL, we found that a) the average rate of LTL attrition was respectively, 27 bp/year (P < 0.001) and 31 bp/year (P < 0.001) for the cross-sectional and longitudinal evaluations, and b) mean TRFL was 180 bp (95 % CI 43, 320) longer in females than males (P < 0.010). For the TRFL distribution, which captures telomeres of all lengths in the DNA sample, we observed significant shifts with age toward shorter telomeres. Based on the measurement error of the TRFLs, we computed that in the longitudinal evaluation 10.6 % of individuals would manifest LTL elongation over 10 years, assuming a 340 bp attrition during this period. This was not significantly different from the empirical observation of 7.5 % of individuals showing LTL elongation. We conclude that accumulation of ultra-short telomeres in leukocytes of the elderly reflects a shift toward shorter telomeres in the entire telomere distribution. Measurement error is the probable explanation for LTL elongation in longitudinal studies. The online version of this article (doi:10.1007/s10654-013-9780-4) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Farzaneh-Far R;Lin J;Epel E;Lapham K;Blackburn E;Whooley MA
通讯作者：
Whooley MA
影响因子：
4.5
作者：
Nordfjall, Katarina;Svenson, Ulrika;Norrback, Karl-Fredrik;Adolfsson, Rolf;Lenner, Per;Roos, Goran
通讯作者：
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影响因子：
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作者：
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通讯作者：
Lansdorp PM
DOI：
10.1073/pnas.0702703104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Njajou, Omer T.;Cawthon, Richard M.;Hsueh, Wen-Chi
通讯作者：
Hsueh, Wen-Chi
DOI：
10.1111/j.1471-0528.2005.00839.x
发表时间：
2006-03-01
影响因子：
5.8
作者：
Akkad, A;Hastings, R;Williams, B
通讯作者：
Williams, B