Bidirectional Regulation of AdpA(ch) in Controlling the Expression of scnRI and scnRII in the Natamycin Biosynthesis of Streptomyces chattanoogensis L10.
Bidirectional Regulation of AdpA(ch) in Controlling the Expression of scnRI and scnRII in the Natamycin Biosynthesis of Streptomyces chattanoogensis L10.
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DOI：
10.3389/fmicb.2018.00316
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Yu P;Bu QT;Tang YL;Mao XM;Li YQ
AdpA是一种AraC/XylS家族蛋白，已被证明是灰色链霉菌次级代谢和形态分化的关键调节因子。在这里，我们确定AdpAch是AdpA的一个直系同系物，在查塔诺产生链霉菌L10中是一种具有双向调节能力的纳他霉素生物合成的“更高水平”的多效性调节因子。DNase I足迹显示在scnRI-scnRII基因间隔区有6个AdpAch结合位点。将XylE报告基因融合到突变结合位点的scnRI-scnRII基因间隔区，进一步分析表明scnRI和scnRII的表达受控于AdpAch。AdpAch显示出一种双稳定的调节能力，它首先与C和D位点结合，激活两个途径特异性基因scnRI和scnRII的转录，然后再与其他发挥抑制作用的位点结合。当A位和F位在体内发生突变时，纳他霉素的产量分别增加了21%和25%。这些发现表明了一种自动调节机制，其中AdpAch作为纳他霉素生物合成的双向调节的主开关。
AdpA, an AraC/XylS family protein, had been proved as a key regulator for secondary metabolism and morphological differentiation in Streptomyces griseus. Here, we identify AdpAch, an ortholog of AdpA, as a “higher level” pleiotropic regulator of natamycin biosynthesis with bidirectional regulatory ability in Streptomyces chattanoogensis L10. DNase I footprinting revealed six AdpAch-binding sites in the scnRI–scnRII intergenic region. Further analysis using the xylE reporter gene fused to the scnRI–scnRII intergenic region of mutated binding sites demonstrated that the expression of scnRI and scnRII was under the control of AdpAch. AdpAch showed a bi-stable regulatory ability where it firstly binds to the Site C and Site D to activate the transcription of the two pathway-specific genes, scnRI and scnRII, and then binds to other sites where it acts as an inhibitor. When Site A and Site F were mutated in vivo, the production of natamycin was increased by 21% and 25%, respectively. These findings indicated an autoregulatory mechanism where AdpAch serves as a master switch with bidirectional regulation for natamycin biosynthesis.
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影响因子：
3
作者：
Du, Yi-Ling;Chen, Shi-Fei;Li, Yong-Quan
通讯作者：
Li, Yong-Quan
影响因子：
3.6
作者：
RETZLAFF, L;DISTLER, J
通讯作者：
DISTLER, J
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF
影响因子：
3.6
作者：
Pan, Yuanyuan;Liu, Gang;Tan, Huarong
通讯作者：
Tan, Huarong
影响因子：
5.6
作者：
Kato, J;Ohnish, Y;Horinouchi, S
通讯作者：
Horinouchi, S