Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method
Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method
复制标题
一种简单准确的尼罗红荧光法测定不同产油酵母的细胞内脂质含量
DOI：
10.1080/10826068.2019.1587624
复制
发表时间：
2019-03
影响因子：
2.9
通讯作者：
Chen Xin-De
中科院分区：
文献类型：
--
作者：
Zhao Cheng;Luo Mu-Tan;Huang Chao;Chen Xue-Fang;Xiong Lian;Li Hai-Long;Chen Xin-De
Abstract A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 °C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 μg/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production.
登录
查看更多内容
DOI：
10.1016/b978-0-08-101023-5.00010-8
发表时间：
2017
期刊：
--
影响因子：
--
作者：
L. Gouveia;A. C. Oliveira;R. Congestri;L. Bruno;A. T. Soares;R. S. Menezes;N. Filho;I. Tzovenis
通讯作者：
L. Gouveia;A. C. Oliveira;R. Congestri;L. Bruno;A. T. Soares;R. S. Menezes;N. Filho;I. Tzovenis
影响因子：
6.3
作者：
Rumin J;Bonnefond H;Saint-Jean B;Rouxel C;Sciandra A;Bernard O;Cadoret JP;Bougaran G
通讯作者：
Bougaran G
DOI：
10.1007/bf02702101
发表时间：
1983-07
期刊：
Journal of the American Oil Chemists’ Society
影响因子：
--
作者：
S. Yoon;J. Rhee
通讯作者：
S. Yoon;J. Rhee
改进的高通量尼罗红荧光测定法，用于估计各种酵母菌中细胞内脂质。
影响因子：
2.2
作者：
Sitepu, I. R.;Ignatia, L.;Franz, A. K.;Wong, D. M.;Faulina, S. A.;Tsui, M.;Kanti, A.;Boundy-Mills, K.
通讯作者：
Boundy-Mills, K.
影响因子：
6.1
作者：
Carvalho, AP;Malcata, FX
通讯作者：
Malcata, FX