The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae.
The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae.
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DOI：
10.1186/s13068-015-0220-4
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发表时间：
2015
影响因子：
6.3
通讯作者：
Bougaran G
中科院分区：
文献类型：
--
作者：
Rumin J;Bonnefond H;Saint-Jean B;Rouxel C;Sciandra A;Bernard O;Cadoret JP;Bougaran G
微藻目前正成为下一代食品、饲料、化妆品和生物燃料形式的可再生能源中最有前途的替代来源之一。微藻是一种种类繁多的微生物，具有生长速度快、效率高等优点。此外，它们不竞争耕地，提供非常高的脂质产量潜力。开发这一资源的主要挑战是利用高通量染色法选择富含脂质的菌株，以测定微藻物种的中性脂含量。为此，最常用于定量脂质的荧光染料是尼罗红和BODIPY 505/515。他们的脂质荧光染色提供了一种快速和廉价的分析工具来测量中性脂质含量，避免了耗时和昂贵的重量分析。本文综述了藻类脂质染色的最新进展，重点介绍了尼罗河红和BODIPY 505/515染色的特点。现有的文献解决了荧光染料在某些条件下的局限性，如光谱特性、染料浓度、细胞浓度、温度和孵育时间。此外，本综述研究的总体结论指出了使用荧光染料筛选富脂微藻物种的局限性，并提出了改进染色顽固微藻的方案和染色定量建议。本文的在线版本（doi:10.1186/s13068-015-0220-4）包含补充材料，可供授权用户使用。
Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification. The online version of this article (doi:10.1186/s13068-015-0220-4) contains supplementary material, which is available to authorized users.
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影响因子：
2.2
作者：
Chen, Wei;Zhang, Chengwu;Hu, Qiang
通讯作者：
Hu, Qiang
DOI：
10.1051/jbio:2008022
发表时间：
2008-01-01
期刊：
Journal de la Societe de Biologie
影响因子：
--
作者：
Cadoret, Jean-Paul;Bernard, Olivier
通讯作者：
Bernard, Olivier
影响因子：
10.8
作者：
Castro, GR;Kamdar, RR;Kaplan, DL
通讯作者：
Kaplan, DL
影响因子：
2.2
作者：
Brennan, Liam;Fernandez, Alfonso Blanco;Owende, Philip
通讯作者：
Owende, Philip
影响因子：
4.1
作者：
Chen, Zhuo;Gong, Yangmin;Hu, Hanhua
通讯作者：
Hu, Hanhua