MOFzyme: Intrinsic protease-like activity of Cu-MOF.
MOFzyme: Intrinsic protease-like activity of Cu-MOF.
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MOFzyme：Cu-MOF 的固有蛋白酶样活性
DOI：
10.1038/srep06759
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发表时间：
2014-10-24
影响因子：
4.6
通讯作者：
Yuan F
中科院分区：
文献类型：
--
作者：
Li B;Chen D;Wang J;Yan Z;Jiang L;Deliang Duan;He J;Luo Z;Zhang J;Yuan F
由于多肽键的高度稳定性和蛋白水解酶在生物和工业中的重要性，构建高效的模拟酶来降解蛋白质中的多肽键是一项具有挑战性的工作。金属有机骨架(MOF)是由无限晶格、金属簇合物和有机连接物组成的有机骨架，它为模拟未知的蛋白酶提供了机会。在此，我们报道了Cu2(C9H3O6)4/3 MOF(众所周知的HKUST-1，这里简称为Cu-MOF)，具有与天然胰酶对牛血清白蛋白(BSA)和酪蛋白的内源性酶相似的活性。铜-MOF的米氏常数(Km)约为游离胰酶的26,000倍，说明BSA对铜-MOF具有更高的亲和力。与均相人工金属蛋白酶铜(II)络合物相比，铜-MOF具有更高的催化效率，可以重复使用10次而不会失去活性。此外，即使在没有EDTA的情况下，铜-MOF也能在细胞培养中分离细胞，因此成功地用于模拟细胞培养中的胰酶作用。
The construction of efficient enzyme mimetics for the hydrolysis of peptide bonds in proteins is challenging due to the high stability of peptide bonds and the importance of proteases in biology and industry. Metal-organic frameworks (MOFs) consisting of infinite crystalline lattices with metal clusters and organic linkers may provide opportunities for protease mimic which has remained unknown. Herein, we report that Cu2(C9H3O6)4/3 MOF (which is well known as HKUST-1 and denoted as Cu-MOF here), possesses an intrinsic enzyme mimicking activity similar to that found in natural trypsin to bovine serum albumin (BSA) and casein. The Michaelis constant (Km) of Cu-MOF is about 26,000-fold smaller than that of free trypsin indicating a much higher affinity of BSA for Cu-MOF surface. Cu-MOF also exhibited significantly higher catalytic efficiency than homogeneous artificial metalloprotease Cu(II) complexes and could be reused for ten times without losing in its activity. Moreover, Cu-MOF was successfully used to simulate trypsinization in cell culture since it dissociated cells in culture even without EDTA.
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影响因子：
4.6
作者：
Chen, Yao;Lykourinou, Vasiliki;Ma, Shengqian
通讯作者：
Ma, Shengqian
影响因子：
56.9
作者：
Chui, SSY;Lo, SMF;Williams, ID
通讯作者：
Williams, ID
影响因子：
15
作者：
Lykourinou, Vasiliki;Chen, Yao;Ma, Shengqian
通讯作者：
Ma, Shengqian
影响因子：
4.9
作者：
Jung, Suhyun;Kim, Youngmee;Park, Seongsoon
通讯作者：
Park, Seongsoon
影响因子：
15
作者：
Larsen, Randy W.;Wojtas, Lukasz;Vetromile, Carissa M.
通讯作者：
Vetromile, Carissa M.