T cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts.
T cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts.
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DOI：
10.4049/jimmunol.1200850
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发表时间：
2012-08-15
期刊：
影响因子：
--
通讯作者：
Sette A
中科院分区：
文献类型：
--
作者：
Oseroff C;Sidney J;Vita R;Tripple V;McKinney DM;Southwood S;Brodie TM;Sallusto F;Grey H;Alam R;Broide D;Greenbaum JA;Kolla R;Peters B;Sette A
A panel of 133 allergens derived from 28 different sources, including fungi, trees, grasses, weeds and indoor allergens, was surveyed utilizing prediction of HLA class II binding peptides and ELISPOT assays with PBMC from allergic donors, resulting in the identification of 257 T cell epitopes. More than 90% of the epitopes were novel, and for 14 allergen sources were the first ever identified. The epitopes identified in the different allergen sources summed up to a variable fraction of the total extract response. In cases of allergens where the identified T cell epitopes accounted for a minor fraction of the extract response, fewer known protein sequences were available, suggesting that for “low epitope coverage” allergen sources, additional allergen proteins remain to be identified. IL-5 and IFN-γresponses were measured as prototype Th2 and Th1 responses, respectively. While in some cases (e.g., Orchard Grass, Alternaria, Cypress, and Russian Thistle) IL-5 production greatly exceeded IFN-γ, in others (e.g., Aspergillus, Penicillum, and Alder) the production of IFN-γ exceeded IL-5. Thus, different allergen sources are associated with variable polarization of the responding T cells. The present study represents the most comprehensive survey to date of human allergen derived T cell epitopes. These epitopes might be used to characterize T cell phenotype/T cell plasticity as a function of seasonality, or as a result of SIT treatment or varying disease severity (asthma or rhinitis).
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DOI：
10.4049/jimmunol.1103556
发表时间：
2012-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Arlehamn CS;Sidney J;Henderson R;Greenbaum JA;James EA;Moutaftsi M;Coler R;McKinney DM;Park D;Taplitz R;Kwok WW;Grey H;Peters B;Sette A
通讯作者：
Sette A
影响因子：
8.7
作者：
Burton OT;Oettgen HC
通讯作者：
Oettgen HC
影响因子：
5.4
作者：
Botten, Jason;Alexander, Jeff;Buchmeier, Michael J.
通讯作者：
Buchmeier, Michael J.
影响因子：
5.4
作者：
Kotturi, Maya F.;Peters, Bjoern.;Sette, Alessandro
通讯作者：
Sette, Alessandro
影响因子：
32.4
作者：
Barrett, Nora A.;Austen, K. Frank
通讯作者：
Austen, K. Frank