Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly.
Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly.
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DOI：
10.1016/j.celrep.2023.113037
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发表时间：
2023-09-26
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
上丘(SC)是中脑中的一种感觉运动结构，它整合了多种感觉模式的输入来启动运动命令。它在发育过程中经历了很好的电路组装步骤，使小鼠SC成为研究神经连接建立的流行模型。在这里，我们对不同发育时间点分离的小鼠SC进行了单核RNA测序分析。我们的研究提供了一个细胞类型的转录图景，这些细胞类型构成了小鼠发育过程中的SC，特别强调了神经元的异质性。我们报告了一系列不同出生年龄差异表达的基因，其中许多已知调节轴突引导和突触形成。利用这些数据，我们发现Pax7的表达仅限于GABA能神经元的一个子集。我们的数据为询问电路发育的机制和识别操纵特定SC神经元群体和电路的标记提供了宝贵的资源。Choi等人。对不同年龄的小鼠上丘(SC)进行单核RNA-SEQ。结果表明，在发育和成熟的SC中，细胞群体及其转录特征。为了促进开放探索，作者创建了一个门户网站，用于分析不同年龄段的SC基因表达谱。
The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering the mouse SC a popular model to study establishment of neural connectivity. Here we perform single-nucleus RNA-sequencing analysis of the mouse SC isolated at various developmental time points. Our study provides a transcriptomic landscape of the cell types that comprise the SC across murine development with particular emphasis on neuronal heterogeneity. We report a repertoire of genes differentially expressed across the different postnatal ages, many of which are known to regulate axon guidance and synapse formation. Using these data, we find that Pax7 expression is restricted to a subset of GABAergic neurons. Our data provide a valuable resource for interrogating the mechanisms of circuit development and identifying markers for manipulating specific SC neuronal populations and circuits. Choi et al. perform single-nucleus RNA-seq on mouse superior colliculus (SC) at various ages. Results show cell populations and their transcriptional signatures in developing and mature SC. To facilitate open exploration, the authors create a web portal for analyzing SC gene expression profiles across the ages.
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影响因子：
3.5
作者：
Ito S;Feldheim DA
通讯作者：
Feldheim DA
影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
DOI：
10.1073/pnas.1717871115
发表时间：
2018-01-30
影响因子：
11.1
作者：
Kalish BT;Cheadle L;Hrvatin S;Nagy MA;Rivera S;Crow M;Gillis J;Kirchner R;Greenberg ME
通讯作者：
Greenberg ME
DOI：
10.1002/cne.23952
发表时间：
2016-08-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Byun H;Kwon S;Ahn HJ;Liu H;Forrest D;Demb JB;Kim IJ
通讯作者：
Kim IJ