Relevance of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) in the structural integrity of the chromatoid body during spermatogenesis.
Relevance of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) in the structural integrity of the chromatoid body during spermatogenesis.
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DOI：
10.1016/j.bbamcr.2010.02.004
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Dufau ML
中科院分区：
文献类型：
--
作者：
Sato H;Tsai-Morris CH;Dufau ML
促性腺激素调节的睾丸RNA解旋酶（GRTH/DDX 25）是一种多功能蛋白，是核糖核蛋白复合物的组成部分，在精子发生过程中起重要作用。我们研究了GRTH在生殖细胞中的核/质穿梭及其对拟染色体（CB）的影响-一种被视为mRNA储存/加工位点的核周细胞器。GRTH存在于圆形精子细胞的细胞核、细胞质和CB中。用核输出或RNA合成的抑制剂处理这些细胞引起GRTH的核保留及其在细胞质和CB中的缺失。GRTH结合的RNA信息的核水平显着增强，并观察到在细胞质中的主要减少。这表明GRTH主要将mRNA转运到细胞质和CB中。MVH，生殖细胞解旋酶，和MIWI，RNA诱导的沉默复合物（RISC）的一个组成部分，局限于CB/细胞质，是不存在的CB和积累在处理后的细胞质中。这也发生在GRTH-KO小鼠的精子细胞中。处理后CB从小叶丝状变为小的凝聚结构，类似于GRTH-KO的CB。GRTH与蛋白质和RNA中的MVH或RISC成员没有相互作用。GRTH除了参与相关生精基因的信息传递外，还将自身的信息传递到细胞质位点。我们的研究表明，GRTH通过其出口/运输功能作为mRNP的一个组成部分是必不可少的，以管理精子细胞中的CB结构，并保持系统，可能参与mRNA的存储和精子发生过程中的处理。
Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25), a multifunctional protein and a component of ribonucleoprotein complexes, is essential for the completion of spermatogenesis. We investigated the nuclear/cytoplasmic shuttling of GRTH in germ cells and its impact on the chromatoid body (CB) -a perinuclear organelle viewed as a storage/processing site of mRNAs. GRTH resides in the nucleus, cytoplasm and CB of round spermatids. Treatment of these cells with inhibitors of nuclear export or RNA synthesis caused nuclear retention of GRTH and its absence in the cytoplasm and CB. The nuclear levels of GRTH bound RNA messages were significantly enhanced and major reduction was observed in the cytoplasm. This indicated GRTH main transport function of mRNAs to the cytoplasm and CB. MVH, a germ cell helicase, and MIWI, a component of the RNA-induced-silencing complex (RISC), confined to the CB/cytoplasm, were absent in the CB and accumulated in the cytoplasm upon treatment. This also occurred in spermatids of GRTH-KO mice. The CB changed from lobular-filamentous to a small condensed structure after treatment resembling the CB of GRTH-KO. No interaction of GRTH with MVH or RISC members in both protein and RNA were observed. Besides of participating in the transport of messages of relevant spermatogenic genes, GRTH was found to transport its own message to cytoplasmic sites. Our studies suggest that GRTH through its export/transport function as a component of mRNP is essential to govern the CB structure in spermatids and to maintain systems that may participate in mRNA storage and their processing during spermatogenesis.
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DOI：
10.1073/pnas.0509333103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Kotaja, N;Bhattacharyya, SN;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
4.8
作者：
Sheng, Yi;Tsai-Morris, Chon-Hwa;Dufau, Maria L.
通讯作者：
Dufau, Maria L.
影响因子：
2.4
作者：
Yokota S
通讯作者：
Yokota S
影响因子：
4
作者：
Kotaja, Noora;Lin, Haifan;Sassone-Corsi, Paolo
通讯作者：
Sassone-Corsi, Paolo
DOI：
10.1002/jemt.1060190207
发表时间：
1991-10-01
期刊：
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE
影响因子：
--
作者：
TOPPARI, J;KANGASNIEMI, M;PARVINEN, M
通讯作者：
PARVINEN, M