Rapid Cellular-Resolution Skin Imaging with Optical Coherence Tomography Using All-Glass Multifocal Metasurfaces.
Rapid Cellular-Resolution Skin Imaging with Optical Coherence Tomography Using All-Glass Multifocal Metasurfaces.
复制标题
使用全玻璃多焦超表面的光学相干层析快速细胞分辨率皮肤成像。
DOI：
10.1021/acsnano.2c09542
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发表时间：
2023-02-28
期刊：
影响因子：
17.1
通讯作者：
de la Zerda, Adam
中科院分区：
文献类型：
--
作者：
Zhao, Jingjing;Van Vleck, Aidan;Winetraub, Yonatan;Du, Lin;Han, Yong;Aasi, Sumaira;Sarin, Kavita Yang;de la Zerda, Adam
关键词：
细胞分辨率光学相干断层扫描（OCT）是一种强大的工具，提供非侵入性的组织学成像。然而，与其他光学显微镜工具一样，高数值孔径（N.A.）需要透镜产生紧聚焦，从而产生窄景深，这需要动态聚焦并限制成像速度。为了克服这一局限性，我们开发了一种生成多个轴向焦点的元表面平台，通过提供多个焦平面，使体积OCT成像速度成倍增加。该平台可精确灵活地控制轴向焦点的数量、位置和强度。直径为8 mm的全玻璃超颖表面光学元件由熔融石英晶片制成，并应用于我们的扫描OCT系统中。多焦点OCT在所有深度上的恒定横向分辨率为1.1 μm，使皮肤病成像的体积采集速度提高了三倍，同时仍然清晰地显示角质层、表皮细胞和真皮-表皮连接的特征，并提供形态学信息作为基底细胞癌的诊断标准。成像速度可以在稀疏样本中进一步提高，例如，7-用七焦点光束折叠总之，这项工作展示了基于超表面的多焦点OCT快速虚拟活检的概念，进一步为开发具有高分辨率和紧凑体积的快速体积成像系统提供了见解。
Cellular-resolution optical coherence tomography (OCT) is a powerful tool offering noninvasive histology-like imaging. However, like other optical microscopy tools, a high numerical aperture (N.A.) lens is required to generate a tight focus, generating a narrow depth of field, which necessitates dynamic focusing and limiting the imaging speed. To overcome this limitation, we developed a metasurface platform that generates multiple axial foci, which multiplies the volumetric OCT imaging speed by offering several focal planes. This platform offers accurate and flexible control over the number, positions, and intensities of axial foci generated. All-glass metasurface optical elements 8 mm in diameter are fabricated from fused-silica wafers and implemented into our scanning OCT system. With a constant lateral resolution of 1.1 μm over all depths, the multifocal OCT triples the volumetric acquisition speed for dermatological imaging, while still clearly revealing features of stratum corneum, epidermal cells, and dermal-epidermal junctions and offering morphological information as diagnostic criteria for basal cell carcinoma. The imaging speed can be further improved in a sparse sample, e.g., 7-fold with a seven-foci beam. In summary, this work demonstrates the concept of metasurface-based multifocal OCT for rapid virtual biopsy, further providing insights for developing rapid volumetric imaging systems with high resolution and compact volume.
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