Phenotyping sensory nerve endings in vitro in the mouse.
Phenotyping sensory nerve endings in vitro in the mouse.
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DOI：
10.1038/nprot.2008.223
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发表时间：
2009
期刊：
影响因子：
14.8
通讯作者：
Reeh PW
中科院分区：
文献类型：
--
作者：
Zimmermann K;Hein A;Hager U;Kaczmarek JS;Turnquist BP;Clapham DE;Reeh PW
本方案详细说明了在离体哺乳动物皮肤隐神经制备中识别和记录皮肤初级传入轴突的方法。该方法是基于从单纤维感受野传播动作电位的细胞外记录。皮肤神经末梢对各种刺激形式表现出分级的敏感性，这些刺激形式通过用热、冷、触摸、恒定点状压力或化学品对灌流皮肤进行充分和受控的刺激来量化。从单纤维记录的响应与以前在同一物种的体内实验中获得的那些是可比的。我们描述了皮肤神经记录站的基本设备的组成和设置（几天），小鼠皮肤和粘附隐神经的制备（15-45分钟）以及神经元的分离和记录（每次记录约1-3小时）。此外，刺激技术，协议，以实现单纤维记录，数据采集和动作电位歧视的问题进行了详细讨论。
This protocol details methods to identify and record from cutaneous primary afferent axons in an isolated mammalian skin–saphenous nerve preparation. The method is based on extracellular recordings of propagated action potentials from single-fiber receptive fields. Cutaneous nerve endings show graded sensitivities to various stimulus modalities that are quantified by adequate and controlled stimulation of the superfused skin with heat, cold, touch, constant punctate pressure or chemicals. Responses recorded from single-fibers are comparable with those obtained in previous in vivo experiments on the same species. We describe the components and the setting-up of the basic equipment of a skin–nerve recording station (few days), the preparation of the skin and the adherent saphenous nerve in the mouse (15–45 min) and the isolation and recording of neurons (approximately 1–3 h per recording). In addition, stimulation techniques, protocols to achieve single-fiber recordings, issues of data acquisition and action potential discrimination are discussed in detail.
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影响因子：
5.5
作者：
Brock, JA;McLachlan, EM;Belmonte, C
通讯作者：
Belmonte, C
影响因子：
5.5
作者：
CATTON, WT
通讯作者：
CATTON, WT
DOI：
10.1016/0024-3205(69)90283-5
发表时间：
1969-01-01
期刊：
LIFE SCIENCES PART 1 PHYSIOLOGY AND PHARMACOLOGY AND PART 2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY
影响因子：
--
作者：
BRETAG, AH
通讯作者：
BRETAG, AH
影响因子：
4.7
作者：
Benedikt, Jan;Teisinger, Jan;Vlachova, Viktorie
通讯作者：
Vlachova, Viktorie
影响因子：
16.2
作者：
Colburn, Raymond W.;Lubin, Mary Lou;Qin, Ning
通讯作者：
Qin, Ning