mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer.
mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer.
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DOI：
10.1186/1476-4598-6-54
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发表时间：
2007-08-23
期刊：
影响因子：
37.3
通讯作者：
Negrini M
中科院分区：
文献类型：
--
作者：
Lanza G;Ferracin M;Gafà R;Veronese A;Spizzo R;Pichiorri F;Liu CG;Calin GA;Croce CM;Negrini M
结直肠癌通过两种主要的遗传不稳定途径发展，其特征在于不同的病理特征和临床结果。我们研究了结肠癌样本（23例具有微卫星稳定性（MSS），16例具有高微卫星不稳定性（MSI-H））的microRNA（miRNA）和mRNA的全基因组表达。基于组合的miRNA和mRNA基因表达，由27个差异表达基因组成的分子标记（包括8个miRNA）可以正确区分MSI-H与MSS结肠癌样品。在差异表达的miRNA中，致癌miR-17-92家族的各种成员在MSS癌症中显著上调。大多数蛋白质编码基因也在MSS癌症中上调。它们的功能分类显示，它们最常与细胞周期、DNA复制、重组、修复、胃肠道疾病和免疫应答相关。这是第一份报告表明MSS与MSI-H结直肠癌之间存在miRNA表达差异。此外，这项工作表明，mRNA/miRNA表达特征的组合可能代表了改善人类癌症生物分子分类的一般方法。
Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
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37.3
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Banerjea A;Ahmed S;Hands RE;Huang F;Han X;Shaw PM;Feakins R;Bustin SA;Dorudi S
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作者：
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通讯作者：
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Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
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