Direct differentiation of whole blood for forensic serology analysis by thread spray mass spectrometry.
Direct differentiation of whole blood for forensic serology analysis by thread spray mass spectrometry.
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DOI：
10.1039/d0an00857e
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发表时间：
2020-08-21
期刊：
影响因子：
--
通讯作者：
Badu-Tawiah AK
中科院分区：
文献类型：
--
作者：
Jackson S ;Frey BS ;Bates MN ;Swiner DJ ;Badu-Tawiah AK
通过线喷雾质谱 (MS) 可以直接分析沾有血迹的纺织品上的全血。此功能满足了调查人员在犯罪现场调查中的首要任务，即确定污迹是否是血迹。这种线喷雾方法探索了使用证据织物线直接从纺织品内的全血中快速测定血红蛋白，无需事先提取步骤。线喷雾 MS 方法可提供多种信息，与目前假定的 Bluestar® 方法不同，后者能够从单一血液样本中检测血红蛋白（α 链和 β 链）、血红素辅因子和脂质。研究发现，人类、犬科动物和马的血液样本的脂质成分有所不同。丝线喷雾 MS 方法作为法医分析平台的稳健性通过三种方式进行评估：1) 其成功适用于之前通过 Bluestar® 推定方法测试的样品，无需事先对样品进行预处理即可提供验证性测试；2) 成功检测到先前洗涤过的织物中的血红素，这证明了丝线喷雾方法前所未有的选择性；3) 能够分析在环境条件下保存长达 30 天的样品。这些结果证明了丝线喷雾质谱平台在法医血清学方面的潜在能力，及其在证据服装直接分析中的应用，具有快速分析和降低纺织品上血液的假阳性和阴性识别率的优势。
Direct analysis of whole blood on bloodstained textiles is achieved with thread spray mass spectrometry (MS). This capability satisfies investigators’ first priority in crime scene investigations, which is determining if a stain is blood. This thread spray method explores the use of evidentiary fabric threads for rapid determination of hemoglobin directly from whole blood within textiles without prior extraction steps. The multiplicity of information that can be derived from the thread spray MS method distinguishes it from the current presumptive Bluestar® method, by enabling the detection of hemoglobin (both α- and β-chains), the heme co-factor and lipids all from a single blood sample. Lipid composition was found to differ for blood samples originating from human, canine, and horse species. The robustness of the thread spray MS method as a forensic analytical platform was evaluated in three ways: 1) its successful applicability to samples previously tested by the Bluestar® presumptive method, offering a confirmatory test without prior sample pre-treatment, 2) successful detection of heme from previously washed fabrics, which demonstrated the unprecedented selectivity of the thread spray method, and 3) the ability to analyze samples stored under ambient conditions for up to 30-days. These results attest to the potential capabilities of the thread spray MS platform in forensic serology, and its application for direct analysis of evidentiary garments, which confer the advantages of rapid analysis and the reduction of the false positive and negative identification rates for blood on textiles.
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影响因子：
16.6
作者：
Gomez-Rios, German Augusto;Pawliszyn, Janusz
通讯作者：
Pawliszyn, Janusz
影响因子：
6.1
作者：
Coon, Allix M.;Beyramysoltan, Samira;Musah, Rabi A.
通讯作者：
Musah, Rabi A.
影响因子：
2
作者：
Musah, Rabi A.;Cody, Robert B.;Shepard, Jason R. E.
通讯作者：
Shepard, Jason R. E.
影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
影响因子：
6.1
作者：
Castello, Ana;Frances, Francesc;Verdu, Fernando
通讯作者：
Verdu, Fernando