Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing.
Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing.
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DOI：
10.1093/nar/gkr1229
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发表时间：
2012-04
影响因子：
14.9
通讯作者：
Kurumizaka H
中科院分区：
文献类型：
--
作者：
Morozumi Y;Ino R;Takaku M;Hosokawa M;Chuma S;Kurumizaka H
PSF 被认为在 RNA 加工、转录和有丝分裂重组 DNA 修复中具有多种功能。在本研究中，我们发现PSF是在精原细胞、精母细胞和精子细胞中产生的，这表明PSF也可能在减数分裂重组中发挥作用。我们测试了 PSF 对减数分裂特异性重组酶 DMC1 同源配对的影响，发现人类 PSF 强烈刺激它。 PSF 在同源配对过程中协同增强含有 DMC1、ssDNA 和 dsDNA 的突触复合体的形成。 PSF 介导的 DMC1 刺激可能是通过其 DNA 聚集活性来促进的，这增加了 DMC1 进行同源配对的 ssDNA 和 dsDNA 的局部浓度。这些结果表明，PSF 可能在高等真核生物中充当减数分裂特异性重组酶 DMC1 的激活剂。
PSF is considered to have multiple functions in RNA processing, transcription and DNA repair by mitotic recombination. In the present study, we found that PSF is produced in spermatogonia, spermatocytes and spermatids, suggesting that PSF may also function in meiotic recombination. We tested the effect of PSF on homologous pairing by the meiosis-specific recombinase DMC1, and found that human PSF robustly stimulated it. PSF synergistically enhanced the formation of a synaptic complex containing DMC1, ssDNA and dsDNA during homologous pairing. The PSF-mediated DMC1 stimulation may be promoted by its DNA aggregation activity, which increases the local concentrations of ssDNA and dsDNA for homologous pairing by DMC1. These results suggested that PSF may function as an activator for the meiosis-specific recombinase DMC1 in higher eukaryotes.
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影响因子：
64.8
作者：
Cox, MM;Goodman, MF;Marians, KJ
通讯作者：
Marians, KJ
DOI：
10.1073/pnas.82.17.5646
发表时间：
1985-01-01
影响因子：
11.1
作者：
CHOW, SA;RADDING, CM
通讯作者：
RADDING, CM
影响因子：
14.9
作者：
Kovalenko, OV;Golub, EI;Radding, CM
通讯作者：
Radding, CM
影响因子：
16.8
作者：
Haruta, Nami;Kurokawa, Yumiko;Iwasaki, Hiroshi
通讯作者：
Iwasaki, Hiroshi
影响因子：
3.6
作者：
Kuwahara, Sho;Ikei, Asako;Kurihara, Yasuyuki
通讯作者：
Kurihara, Yasuyuki