Mimotope vaccination for epitope-specific induction of anti-VEGF antibodies.
Mimotope vaccination for epitope-specific induction of anti-VEGF antibodies.
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DOI：
10.1186/1472-6750-13-77
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发表时间：
2013-09-27
影响因子：
3.5
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Li W;Ran Y;Li M;Zhang K;Qin X;Xue X;Zhang C;Hao Q;Zhang W;Zhang Y
肿瘤血管生成是肿瘤生长、浸润和转移的关键。血管内皮生长因子（VEGF）是一种有效的血管生成因子，靶向它对减少血管生成具有重要意义。贝伐单抗（Avastin）是一种直接作用于VEGF的单克隆抗体，已被证明对多种癌症如直肠癌、结肠癌、非小细胞肺癌等有有效的治疗效果。在目前的研究中，我们使用噬菌体展示技术生成了与筛选的阿瓦斯汀抗体（Ab）互补的模位。从一个12聚肽文库中分离出候选的VEGF模位。显示DHTLYTPYHTHP肽（标记为12P）的噬菌体对Avastin具有高亲和力。用戊二醛（GA）将化学合成的12P与锁孔帽贝血青素（KLH）偶联，形成疫苗KLH-12肽（KLH-12P）。该抗原表位疫苗可显著诱导小鼠体液免疫。klh - 12p免疫小鼠血清与VEGF相关，阻断其与VEGFR的结合，从而抑制血管内皮细胞的增殖和迁移。我们的数据表明，这里报道的分离的mimotope 12P可能会引发针对VEGF的特异性抗体，并导致诱导抗血管生成反应。
Tumor angiogenesis is critical for tumor growth, infiltration and metastasis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and targeting it is important in reducing angiogenesis. Bevacizumab (Avastin), a monoclonal antibody that reacts directly against VEGF, has been demonstrated to be an effective treatment for various cancers such as rectal cancer, colon carcinoma, and non-small cell lung cancer, etc. In the current study, we used the phage display technique to generate mimotopes that complemented the screening Avastin antibody (Ab). The candidate mimotopes of VEGF were isolated from a 12-mer peptide library. The phage displaying peptide DHTLYTPYHTHP (designated as 12P) exhibited high affinity to Avastin. The chemically synthesized 12P was conjugated to keyhole limpet hemocyanin (KLH) by glutaraldehyde (GA) to form vaccine KLH-12 peptide (KLH-12P). This epitope vaccine significantly induced humoral immunity in mice. The blood serum from KLH-12P-immunized mice associated with VEGF and blocked its binding to VEGFR, thus inhibiting vascular endothelial cell proliferation and migration. Our data indicate that the isolated mimotope 12P reported here could potentially elicit specific antibodies against VEGF and result in the induction of anti-angiogenesis responses.
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影响因子：
4.4
作者：
Riemer, Angelika B.;Jensen-Jarolim, Erika
通讯作者：
Jensen-Jarolim, Erika
影响因子：
46.9
作者：
De Berardinis, P;Sartorius, R;Guardiola, J
通讯作者：
Guardiola, J
DOI：
10.2165/00063030-200721030-00002
发表时间：
2007
期刊：
BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy
影响因子：
--
作者：
Gershoni JM;Roitburd-Berman A;Siman-Tov DD;Tarnovitski Freund N;Weiss Y
通讯作者：
Weiss Y
用针对幽门螺杆菌脲酶 B 的中和抗体选择的模拟表位在接种疫苗后在小鼠中诱导酶抑制抗体
影响因子：
3.5
作者：
Li Y;Ning Y;Wang Y;Peng D;Jiang Y;Zhang L;Long M;Luo J;Li M
通讯作者：
Li M
影响因子：
3.1
作者：
Larralde, Osmany G.;Martinez, Raiza;Perez, Ela M.
通讯作者：
Perez, Ela M.