Isolation and purification of a peptide from Bullacta exarata and its impaction of apoptosis on prostate cancer cell.
Isolation and purification of a peptide from Bullacta exarata and its impaction of apoptosis on prostate cancer cell.
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DOI：
10.3390/md11010266
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发表时间：
2013-01-23
期刊：
影响因子：
5.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Ma J;Huang F;Lin H;Wang X
用胰蛋白酶水解泥螺制备肽；通过超滤分离水解产物并使用G-25凝胶过滤纯化。通过HPLC验证了泥螺的纯度并对其肽序列进行了分析。使用MTT测定检测BEPT II和BEPT II-1对PC-3细胞增殖的影响。 BEPT II和BEPT II-1以时间和剂量依赖性方式显着抑制PC-3细胞的增殖。 Annexin V/PI 双染色研究表明，将 PC-3 细胞暴露于 5 或 15 mg/mL BEPT II-1 24 小时，早期凋亡细胞的百分比从 11.22% 增加至 22.09%。此外，吖啶橙/溴化乙锭染色观察到细胞典型的形态变化。这些数据支持BEPT II-1具有抗癌特性，值得进一步研究以了解BEPT II-1诱导PC-3细胞凋亡的机制。
Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%. In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. These data support that BEPT II-1 has anticancer properties and merits further investigation to understand the mechanisms of BEPT II-1-induced apoptosis in PC-3 cells.
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影响因子：
56.9
作者：
Pan, GH;ORourke, K;Dixit, VM
通讯作者：
Dixit, VM
影响因子：
4.8
作者：
Zapata, JM;Pawlowski, K;Reed, JC
通讯作者：
Reed, JC
影响因子：
3.5
作者：
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影响因子：
4.3
作者：
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通讯作者：
Balasubramanian, Maruthaiveeran Periyasamy
影响因子：
2.1
作者：
Gilligan, T;Kantoff, PW
通讯作者：
Kantoff, PW