Interactional mechanisms of Paenibacillus polymyxa SC2 and pepper (Capsicum annuum L.) suggested by transcriptomics.
Interactional mechanisms of Paenibacillus polymyxa SC2 and pepper (Capsicum annuum L.) suggested by transcriptomics.
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转录组学揭示多粘类芽孢杆菌 SC2 与辣椒 (Capsicum annuum L.) 的相互作用机制
DOI：
10.1186/s12866-021-02132-2
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发表时间：
2021-03-04
期刊：
影响因子：
4.2
通讯作者：
Ding Y
中科院分区：
文献类型：
--
作者：
Liu H;Li Y;Ge K;Du B;Liu K;Wang C;Ding Y
多粘类芽孢杆菌（PaenibacilluspolymyxaSC 2）是从辣椒根际土壤中分离到的一株细菌，促进辣椒生长和生物防治。但P. polymyxaSC 2和胡椒尚未被阐明。本研究旨在探讨P.结果P.在温室盆栽试验中，polymyxaSC 2促进辣椒茎叶生长。在相互作用的条件下，辣椒刺激与群体感应，趋化性和生物膜形成相关的基因的表达。多黏菌SC 2.辣椒诱导多粘菌素和杀镰孢菌素生物合成基因的表达。polymyxaSC 2的表达分别上调2.93- 6.13倍和2.77- 7.88倍。在辣椒培养基的刺激下，P. polymyxaSC 2对柑橘黄单胞菌的抑制作用显著增强。同时，在P. polymyxaSC 2，转录因子WRKY 2和WRKY 40在辣椒中的表达分别上调了1.17倍和3.5倍。p.polymyxaSC2还通过刺激相应转录调控因子的表达诱导辣椒的系统抗性。辣椒对P.多黏菌SC 2.本研究为进一步研究P. polymyxaSC 2和胡椒粉。
BackgroundPaenibacillus polymyxaSC2, a bacterium isolated from the rhizosphere soil of pepper (Capsicum annuumL.), promotes growth and biocontrol of pepper. However, the mechanisms of interaction betweenP. polymyxaSC2 and pepper have not yet been elucidated. This study aimed to investigate the interactional relationship ofP. polymyxaSC2 and pepper using transcriptomics.ResultsP. polymyxaSC2 promotes growth of pepper stems and leaves in pot experiments in the greenhouse. Under interaction conditions, peppers stimulate the expression of genes related to quorum sensing, chemotaxis, and biofilm formation inP. polymyxaSC2. Peppers induced the expression of polymyxin and fusaricidin biosynthesis genes inP. polymyxaSC2, and these genes were up-regulated 2.93- to 6.13-fold and 2.77- to 7.88-fold, respectively. Under the stimulation of medium which has been used to culture pepper, the bacteriostatic diameter ofP. polymyxaSC2 againstXanthomonas citriincreased significantly. Concurrently, under the stimulation ofP. polymyxaSC2, expression of transcription factor genesWRKY2andWRKY40in pepper was up-regulated 1.17-fold and 3.5-fold, respectively.ConclusionsThrough the interaction with pepper, the ability ofP. polymyxaSC2 to inhibit pathogens was enhanced.P. polymyxaSC2 also induces systemic resistance in pepper by stimulating expression of corresponding transcription regulators. Furthermore, pepper has effects on chemotaxis and biofilm formation ofP. polymyxaSC2. This study provides a basis for studying interactional mechanisms ofP. polymyxaSC2 and pepper.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.1
作者：
Devi, Phazna T. A.;Sahoo, Dinabandhu;Devi, Indira S.
通讯作者：
Devi, Indira S.
影响因子：
4
作者：
Castagno, L. N.;Estrella, M. J.;Ruiz, O. A.
通讯作者：
Ruiz, O. A.
影响因子：
4.2
作者：
Latha, P.;Anand, T.;Samiyappan, R.
通讯作者：
Samiyappan, R.
影响因子：
4.9
作者：
Chaney, Rufus L.;Chen, Kuang-Yu;Baker, Alan J. M.
通讯作者：
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