Neurotensin induces Fos and Zif268 expression in limbic nuclei of the rat brain
Neurotensin induces Fos and Zif268 expression in limbic nuclei of the rat brain
复制标题
神经降压素诱导大鼠脑边缘核中 Fos 和 Zif268 的表达
DOI：
10.1016/0306-4522(96)00210-2
复制
发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
C. Kilts
中科院分区：
文献类型：
--
作者：
P. Lambert;T. Ely;R. Gross;C. Kilts
内源性三肽神经紧张素直接注入大脑后，会产生广泛的行为、电生理和神经化学效应。这些效应被认为是由于分布在整个中枢神经系统的神经紧张素受体的不同种群的激活。我们利用直接早期基因c-fos和zif268的核蛋白产物作为细胞激活的标记物，绘制了与神经紧张素中枢给药相关的大鼠脑功能变化位点。大鼠侧脑室注入神经紧张素后，杏仁核中央核和基底核以及下丘脑室旁核和视上核中Fos和Zif268免疫反应阳性的核数量增加。神经紧张素还引起血清皮质酮浓度升高和体温降低。腹腔注射非多肽神经紧张素受体拮抗剂SR48692可阻断神经紧张素诱导的皮质酮分泌，并显著减少杏仁核复合体中神经紧张素诱导的fos阳性和zif268阳性神经元的数量。在杏仁核中央核或基底外侧核中fos阳性核的数量与血清皮质酮浓度之间存在显著的正相关。治疗后下丘脑室旁核zif阳性细胞数量与体温变化呈显著正相关。我们的研究结果表明，神经紧张素在增加血清皮质酮中的核心作用涉及杏仁核中央和基底外侧核Fos的诱导。相比之下，神经紧张素诱导的低温，不受SR48692预处理的影响，涉及下丘脑室旁核的Zif诱导。这些数据进一步支持中枢神经紧张素受体亚型的存在，它可能调节不同的即时早期基因。
The endogenous tridecapeptide neurotensin exerts a wide range of behavioral, electrophysiological and neurochemical effects when administered directly into the brain. These effects are thought to result from the activation of distinct populations of neurotensin receptors distributed throughout the central nervous system. We have mapped the sites of functional change in the rat brain associated with the central administration of neurotensin using the induction of the nuclear protein products of the immediate early genes c-fos and zif268 as markers of cellular activation. The administration of neurotensin into the lateral ventricle of rats produced an increase in the number of nuclei positive for Fos and Zif268 immunoreactivity in the central and basolateral nuclei of the amygdala and the paraventricular and supraoptic nuclei of the hypothalamus. Neurotensin also produced an increase in serum corticosterone concentration and decrease in body temperature. The intraperitoneal administration of SR48692, a non-peptide neurotensin receptor antagonist, blocked the neurotensin-induced corticosterone secretion and significantly reduced the number of neurotensin-induced Fos-positive and Zif268-positive neurons in the amygdaloid complex. A significant positive correlation wash found between the number of Fos-positive nuclei in the central or basolateral nucleus of the amygdala and the serum corticosterone concentration. A significant positive correlation was also found between the number of Zif-positive cells in the paraventricular nucleus of the hypothalamus and change in body temperature following treatment. Our findings indicate that the central role of neurotensin in increasing serum corticosterone involves the induction of Fos in the central and basolateral nuclei of the amygdala. In contrast, the neurotensin-induced hypothermia, which was unaffected by pretreatment with SR48692, involves Zif induction in the paraventricular nucleus of the hypothalamus. These data support further the existence of central neurotensin receptor subtypes which may regulate distinct immediate early genes.
DOI：
10.1073/pnas.87.17.6912
发表时间：
1990-09-01
影响因子：
11.1
作者：
GRAYBIEL, AM;MORATALLA, R;ROBERTSON, HA
通讯作者：
ROBERTSON, HA
DOI：
10.1073/pnas.86.22.8737
发表时间：
1989-11-01
影响因子：
11.1
作者：
CHRISTY, B;NATHANS, D
通讯作者：
NATHANS, D