Introgression in the genus Campylobacter: generation and spread of mosaic alleles.
Introgression in the genus Campylobacter: generation and spread of mosaic alleles.
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DOI：
10.1099/mic.0.045153-0
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Maiden MCJ
中科院分区：
文献类型：
--
作者：
Sheppard SK;McCarthy ND;Jolley KA;Maiden MCJ
水平遗传交换强烈地影响许多细菌的进化，实质上导致难以确定它们在分类群中的位置。特别是，相关细菌基因型的集群-目前被归类为微生物物种-如何进化和维持仍然存在争议。两个密切相关的基因交换的性质和幅度（约。15%核苷酸分歧）微生物定义的菌种，空肠弯曲杆菌和结肠弯曲杆菌，通过检查嵌合等位基因，即来自每个种群的一些祖先进行研究。 共1738个等位基因从2953个七位点管家基因序列类型（ST）的概率分配到每个物种组与基于模型的聚类算法结构。使用结构连锁模型，将分配概率小于75%的等位基因确认为嵌合体。对于这些中的每一个，确定重组区域的推定来源，并将等位基因映射到源自串联ST序列的克隆框架谱系上。这使得两个群体间渐渗的方向和频率得以确立，其中C. coli进化枝1等位基因，具有获得性C.空肠序列，相比之下，0.5%的倒数过程。 一旦产生，嵌合基因在C. coli进化枝1的克隆扩增和横向基因转移相结合，与一些证据的侵蚀镶嵌的重新收购C。coli序列。这些观察结果证实了以前的分析交换完整的管家等位基因，并通过描述受体物种内的水平基因转移和随后的传播过程来扩展这项工作。
Horizontal genetic exchange strongly influences the evolution of many bacteria, substantially contributing to difficulties in defining their position in taxonomic groups. In particular, how clusters of related bacterial genotypes – currently classified as microbiological species – evolve and are maintained remains controversial. The nature and magnitude of gene exchange between two closely related (approx. 15 % nucleotide divergence) microbiologically defined species, Campylobacter jejuni and Campylobacter coli, was investigated by the examination of mosaic alleles, those with some ancestry from each population. A total of 1738 alleles from 2953 seven-locus housekeeping gene sequence types (STs) were probabilistically assigned to each species group with the model-based clustering algorithm structure. Alleles with less than 75 % assignment probability to one of the populations were confirmed as mosaics using the structure linkage model. For each of these, the putative source of the recombinant region was determined and the allele was mapped onto a clonalframe genealogy derived from concatenated ST sequences. This enabled the direction and frequency of introgression between the two populations to be established, with 8.3 % of C. coli clade 1 alleles having acquired C. jejuni sequence, compared to 0.5 % for the reciprocal process. Once generated, mosaic genes spread within C. coli clade 1 by a combination of clonal expansion and lateral gene transfer, with some evidence of erosion of the mosaics by reacquisition of C. coli sequence. These observations confirm previous analyses of the exchange of complete housekeeping alleles and extend this work by describing the processes of horizontal gene transfer and subsequent spread within recipient species.
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影响因子：
3.3
作者：
Didelot, Xavier;Falush, Daniel
通讯作者：
Falush, Daniel
DOI：
10.1073/pnas.92.23.10535
发表时间：
1995-11-07
影响因子：
11.1
作者：
FEIL, E;CARPENTER, G;SPRATT, BG
通讯作者：
SPRATT, BG
影响因子：
3.2
作者：
Caro-Quintero, Alejandro;Rodriguez-Castano, Gina P.;Konstantinidis, Konstantinos T.
通讯作者：
Konstantinidis, Konstantinos T.
影响因子：
3.7
作者：
Friis C;Wassenaar TM;Javed MA;Snipen L;Lagesen K;Hallin PF;Newell DG;Toszeghy M;Ridley A;Manning G;Ussery DW
通讯作者：
Ussery DW
影响因子：
5.4
作者：
Hanage WP;Fraser C;Spratt BG
通讯作者：
Spratt BG