Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis.
Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis.
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生长素通过单翅目起作用，调节植物细胞极性和模式植物。
DOI：
10.1016/j.cub.2016.09.044
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发表时间：
2016-12-05
期刊：
影响因子：
9.2
通讯作者：
Heisler, Marcus G.
中科院分区：
文献类型：
--
作者：
Bhatia, Neha;Bozorg, Behruz;Larsson, Andre;Ohno, Carolyn;Jonsson, Henrik;Heisler, Marcus G.
植物器官（如叶子和花）的周期性形成产生了复杂的图案，数百年来一直吸引着生物学家和数学家。植物激素生长素在建立这些模式中起着核心作用，通过促进器官形成的网站，它积累由于其极性，细胞到细胞的运输。虽然实验证据以及建模表明，反馈生长素的运输方向可能有助于指定叶序模式，这种反馈的性质仍然不清楚。在这里，我们发现，极化的生长素外排载体PIN-FORMED 1（PIN 1）的生长素反应转录因子MONOPTEROS（MP）的调节。我们发现，在拍摄，细胞极性模式遵循MP的表达，这反过来又遵循生长素的分布模式。通过扰动MP活动在全球和本地，我们表明，本地化的MP活动是必要的极性收敛模式的产生和本地化的MP表达是足以指示PIN 1极性方向非细胞自主，对MP表达细胞。通过在MP突变体的表皮中表达MP，我们进一步表明，虽然MP在单细胞层中的活性足以促进极性会聚模式，但MP在表皮下组织中有助于将这些极性模式锚到底层细胞。总的来说，我们的研究结果揭示了植物中的一个模式模块，该模块通过将激素生长素定向运输到具有高水平MP介导的生长素信号的细胞来确定器官位置。我们建议，这种反馈过程广泛地产生周期性的植物结构。生长素调节的MP表达和活性预测PIN 1极性在SAM的变化局部MP活性是必要的，以介导周期性器官形成MP定向PIN 1极性非细胞自主促进局部生长素积累表皮下MP活性是必要的，以稳定生长素的分布模式植物中的器官定位取决于激素生长素到器官起始位点的极性运输。Bhatia等人揭示了生长素反应因子MONOPTEROS自主地定向生长素流出载体PIN 1非细胞的极性，从而促进导致周期性器官形成的正反馈回路。
The periodic formation of plant organs such as leaves and flowers gives rise to intricate patterns that have fascinated biologists and mathematicians alike for hundreds of years. The plant hormone auxin plays a central role in establishing these patterns by promoting organ formation at sites where it accumulates due to its polar, cell-to-cell transport. Although experimental evidence as well as modeling suggest that feedback from auxin to its transport direction may help specify phyllotactic patterns, the nature of this feedback remains unclear. Here we reveal that polarization of the auxin efflux carrier PIN-FORMED 1 (PIN1) is regulated by the auxin response transcription factor MONOPTEROS (MP). We find that in the shoot, cell polarity patterns follow MP expression, which in turn follows auxin distribution patterns. By perturbing MP activity both globally and locally, we show that localized MP activity is necessary for the generation of polarity convergence patterns and that localized MP expression is sufficient to instruct PIN1 polarity directions non-cell autonomously, toward MP-expressing cells. By expressing MP in the epidermis of mp mutants, we further show that although MP activity in a single-cell layer is sufficient to promote polarity convergence patterns, MP in sub-epidermal tissues helps anchor these polarity patterns to the underlying cells. Overall, our findings reveal a patterning module in plants that determines organ position by orienting transport of the hormone auxin toward cells with high levels of MP-mediated auxin signaling. We propose that this feedback process acts broadly to generate periodic plant architectures. Auxin-regulated MP expression and activity predict PIN1 polarity changes at the SAM Localized MP activity is necessary to mediate periodic organ formation MP orients PIN1 polarity non-cell autonomously to promote local auxin accumulation Sub-epidermal MP activity is required to stabilize auxin distribution patterns Organ positioning in plants depends on polar transport of the hormone auxin to organ initiation sites. Bhatia et al. reveal that the auxin response factor MONOPTEROS orients the polarity of the auxin efflux carrier PIN1 non-cell autonomously, thereby facilitating a positive feedback loop that results in periodic organ formation.
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影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
9.2
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1073/pnas.0510457103
发表时间：
2006-01-31
影响因子：
11.1
作者：
Smith, RS;Guyomarc'h, S;Prusinkiewicz, P
通讯作者：
Prusinkiewicz, P
影响因子：
4.8
作者：
Heisler, M. G.;Jonsson, H.
通讯作者：
Jonsson, H.
影响因子：
7.2
作者：
Joubès, J;De Schutter, K;De Veylder, L
通讯作者：
De Veylder, L