DNA strand breakage is correlated with unaltered base release after gamma irradiation.
DNA strand breakage is correlated with unaltered base release after gamma irradiation.
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DNA 链断裂与伽马射线照射后未改变的碱基释放相关。
DOI：
10.2307/3579150
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发表时间：
1995
影响因子：
3.4
通讯作者：
A. Chatterjee
中科院分区：
文献类型：
--
作者：
E. Henle;R. Roots;W. Holley;A. Chatterjee
未改变的碱基释放与在pH 7.4的水溶液中γ-辐照的噬菌体PM2 DNA的链断裂相关。通过琼脂糖凝胶电泳法测定DNA链断裂的产率。高效液相色谱法（HPLC）用于测定未改变的核酸碱基的释放。更新了先前报告的HPLC方法。未改变的碱基释放与高达424戈伊的剂量呈线性关系，其中高达0.2%的所有DNA碱基被释放。没有可检测量的未改变的核苷被释放，并且除了未改变的碱基之外，仅观察到从DNA释放的一种其他产物。碱释放产率未反映43%的PM2 GC含量。只有76%的所有提示链断裂似乎与未改变的游离碱的释放有关，由此鸟嘌呤、胞嘧啶、腺嘌呤和胸腺嘧啶的产率分别为提示链断裂产率的9%、27%、18%和22%。在37 ℃下辐射后孵育24小时使链断裂产率增加1.38倍，未改变的碱基释放产率增加1.76倍，使得97%的最终链断裂似乎与未改变的碱基的释放相关，由此鸟嘌呤、胞嘧啶、腺嘌呤和胸腺嘧啶产率分别为最终链断裂产率的10%、36%、23%和28%。这些数据表明，在适当的条件下，几乎每个链断裂都会导致碱基释放。OH自由基攻击导致链断裂和碱释放这些结果的轴承进行了讨论。
Unaltered base release is correlated with strand breakage for gamma-irradiated bacteriophage PM2 DNA in aqueous solution at pH 7.4. The yield of DNA strand breaks is determined by the agarose gel electrophoresis method. High-performance liquid chromatography (HPLC) is used to assay the release of unaltered nucleic bases. Previously reported HPLC methods have been updated. Unaltered base release is linear with dose up to 424 Gy, where up to 0.2% of all DNA bases are released. No detectable amounts of unaltered nucleosides are released and, besides unaltered bases, only one other product released from DNA is observed. Base release yields do not reflect the PM2 GC content of 43%. Only 76% of all prompt strand breaks appear to be associated with the release of an unaltered free base, whereby the guanine, cytosine, adenine and thymine yields are 9, 27, 18 and 22% of the prompt strand break yield, respectively. Postirradiation incubation at 37 degrees C for 24 h increases the strand break yield 1.38-fold and the unaltered base release yield 1.76-fold such that 97% of the final strand breaks appear to be associated with the release of an unaltered base, whereby the guanine, cytosine, adenine and thymine yields are 10, 36, 23 and 28% of the final strand break yield, respectively. These data indicate that, given proper conditions, nearly every strand break leads to a base release. The bearing of these results on OH radical attack leading to strand breakage and base release is discussed.
辐射后温度对 SV40 DNA 中辐射诱导的单链和双链断裂产量的影响。
影响因子：
3.4
作者：
Jones,GD;Boswell,TV;Ward,JF
通讯作者：
Ward,JF
DOI：
10.1016/s0021-9258(19)70209-9
发表时间：
1980-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Burger;Allen R. Berkowitz;J. Peisach;Susan Band
通讯作者：
R. Burger;Allen R. Berkowitz;J. Peisach;Susan Band
伽马射线照射的 DNA、脱氧核苷酸和脱氧核苷中的丙二醛前体。
影响因子：
14.9
作者：
Janicek,MF;Haseltine,WA;Henner,WD
通讯作者：
Henner,WD