Long lived multi-isotype anti-HIV antibody responses following a prime-double boost immunization strategy.
Long lived multi-isotype anti-HIV antibody responses following a prime-double boost immunization strategy.
复制标题
采用初免-双重加强免疫策略后，可产生长效的多同种型抗 HIV 抗体反应。
DOI：
10.1016/j.vaccine.2004.10.030
复制
发表时间：
2005
期刊：
影响因子：
5.5
通讯作者：
Hurwitz,JL
中科院分区：
文献类型：
--
作者：
Stambas,J;Brown,SA;Gutierrez,A;Sealy,R;Yue,W;Jones,B;Lockey,TD;Zirkel,A;Freiden,P;Brown,B;Surman,S;Coleclough,C;Slobod,KS;Doherty,PC;Hurwitz,JL
Despite decades of work, an effective HIV vaccine remains elusive. In an effort to elicit protective immunity, investigators have sought to define vaccines able to elicit durable HIV-specific B-cell and T-cell activities. Additionally, vaccines are sought which can induce antibodies of a variety of isotypes, as each isotype possesses unique attributes in terms of opsonization, Fc receptor binding capacity, complement fixation and location. One prominent new vaccine strategy, applied to numerous distinct antigenic systems is the prime boost-regimen, with DNA, vaccinia virus (VV), and/or purified recombinant protein. To examine the durability, location and isotype distribution of responses induced by prime-boost regimens, we tested successive immunizations with DNA, VV and protein (D-V-P), comparing three forms of protein inoculations: (i) purified protein administered intramuscularly with complete Freunds adjuvant, (ii) purified protein administered intranasally, and (iii) purified protein conjugated to oxidized mannan, administered intranasally. We found that all three protocols elicited serum antibodies of multiple isotypes, with serum IgA being most prominent among mice immunized with mannan-conjugated protein. All D-V-P protocols, regardless of protein form or route, also elicited antibody responses at mucosal surfaces. In bronchoalveolar lavage, a tendency toward IgA production was again most prominent in mice boosted with the protein–mannan conjugate. Both B-cell and T-cell responses were sustained for more than 1 year post-immunization following each form of vaccination. Contemporaneous with long-lasting serum and mucosal antibodies were antibody forming cells in the bone marrow of primed animals. Results highlight the D-V-P vaccination strategy as a promising approach for attaining durable, multi-isotype B-cell and T-cell activities toward HIV.
登录
查看更多内容
影响因子：
4.4
作者：
D. Marshall;R. Sealy;M. Sangster;C. Coleclough
通讯作者：
D. Marshall;R. Sealy;M. Sangster;C. Coleclough
痘苗病毒载体的制备及其在HIV蛋白表达和免疫中的应用
DOI：
--
发表时间：
1996
期刊：
影响因子：
--
作者：
K. Wryan;R. Jowens;J. Lhurwitz
通讯作者：
J. Lhurwitz
影响因子：
3.7
作者：
Richmond, JFL;Mustafa, F;Robinson, HL
通讯作者：
Robinson, HL
影响因子：
15.9
作者：
Slifka, MK;Ahmed, R
通讯作者：
Ahmed, R
影响因子：
12.7
作者：
Akagi, T;Kawamura, M;Baba, M
通讯作者：
Baba, M