Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer.
Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer.
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DOI：
10.1002/mrm.24641
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发表时间：
2014-01
影响因子：
3.3
通讯作者：
Reddy, Ravinder
中科院分区：
文献类型：
--
作者：
Kogan, Feliks;Haris, Mohammad;Singh, Anup;Cai, Kejia;Debrosse, Catherine;Nanga, Ravi Prakash Reddy;Hariharan, Hari;Reddy, Ravinder
开发基于化学交换饱和转移 (CEST) 的技术来测量游离肌酸 (Cr)，并通过在运动前后以高空间分辨率测量 Cr 在肌肉中的分布来验证该技术。进行模型研究以确定其他肌酸激酶代谢物对 Cr (CrCEST) 的 CEST 效应的贡献。在健康志愿者的跖屈运动前后，使用 7T 全身 MR 扫描仪采集小腿的 CEST、T2、磁化传输比 (MTR) 和 31P 磁共振波谱 (MRS)。模型研究表明，虽然 Cr 表现出显着的 CEST 效应，但其他代谢物没有明显的贡献。在健康人类受试者中，进行轻度跖屈运动后，观察到 CrCEST 增加，并以指数方式恢复到基线。该技术表现出良好的空间分辨率，并且能够区分受试者之间肌肉利用率的差异。 CrCEST 结果与 31P MRS 结果进行比较，显示 Cr 和 PCr 恢复动力学具有良好的一致性。展示了基于 CEST 的技术来测量体内肌肉中游离肌酸的变化。 CrCEST 成像可以在空间上绘制轻度运动后肌肉中肌酸浓度的变化。这可以作为诊断和治疗影响肌肉的各种疾病的工具。
To develop a chemical exchange saturation transfer (CEST) based technique to measure free creatine (Cr) and to validate the technique by measuring the distribution of Cr in muscle with high spatial resolution before and after exercise. Phantom studies were performed to determine contributions from other creatine kinase metabolites to the CEST effect from Cr (CrCEST). CEST, T2, magnetization transfer ratio (MTR) and 31P magnetic resonance spectroscopy (MRS) acquisitions of the lower leg were performed before and after plantar flexion exercise on a 7T whole-body MR scanner on healthy volunteers. Phantom studies demonstrated that while Cr exhibited significant CEST effect there were no appreciable contributions from other metabolites. In healthy human subjects, following mild plantar flexion exercise, increases in CrCEST were observed, which recovered exponentially back to baseline. This technique exhibited good spatial resolution and was able to differentiate differences in muscle utilization among subjects. CrCEST results were compared with 31P MRS results showing good agreement in the Cr and PCr recovery kinetics. Demonstrated a CEST based technique to measure free creatine changes in in vivo muscle. CrCEST imaging can spatially map changes in creatine concentration in muscle following mild exercise. This may serve as a tool for the diagnosis and treatment of various disorders affecting muscle.
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影响因子：
6.7
作者：
FISHER, MJ;MEYER, RA;POTCHEN, EJ
通讯作者：
POTCHEN, EJ
影响因子：
37.8
作者：
MASSIE, BM;CONWAY, M;RADDA, G
通讯作者：
RADDA, G
影响因子：
3.3
作者：
ADAMS, GR;DUVOISIN, MR;DUDLEY, GA
通讯作者：
DUDLEY, GA
影响因子：
3.3
作者：
Rico-Sanz, J;Thomas, EL;Bell, JD
通讯作者：
Bell, JD
影响因子：
2.9
作者：
IOTTI, S;LODI, R;BARBIROLI, B
通讯作者：
BARBIROLI, B