Rapidly evolving genetic features for desert adaptations in Stipagrostis pennata.
Rapidly evolving genetic features for desert adaptations in Stipagrostis pennata.
复制标题
针茅草适应沙漠的快速进化遗传特征
DOI：
10.1186/s12864-021-08124-w
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发表时间：
2021-11-23
期刊：
影响因子：
4.4
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Ding X;Zhang T;Ma L
盘山针茅分布于流动和半流动沙丘中，对干旱、高温等极端环境具有较强的适应能力。它是具有稳定沙丘和生态恢复潜力的先锋植物。它可以比其他沙漠植物更早地在移动的沙丘上定居。它可以有效地提高沙丘的稳定性，帮助更多的植物定居，增加植物多样性。然而，尽管具有重要的生态价值，可用于该物种的遗传资源有限。结果利用单分子实时测序技术获得了pennata stipagrostis完整的全长转录组，包括90,204个单基因，平均长度为2624 bp。此外，在这些单基因中鉴定出的5436个转录因子中含有丰富的抗逆性基因，如myb相关基因、C3H、bHLH、GRAS、HSF等。这可能在适应沙漠干旱和强风胁迫方面发挥作用。内含子保留事件是丰富的备选剪接事件。螺旋藻经历了更强的正选择，加速了优势变异的固定。CPP/ tso1样基因等38个基因进化迅速，可能在物质运输、开花和种子形成中发挥作用。结论本研究获得了羽翼stipagrostis pennata的完整全长转录组，揭示了其快速进化过程。荒漠适应主要体现在对荒漠植物基因表达的调控和对荒漠植物基因功能的适应性。我们的发现为荒漠草物种的进化适应性提供了丰富的知识。
BackgroundStipagrostis pennatais distributed in the mobile and semi-mobile sand dunes which can adapt well to extreme environments such as drought and high temperature. It is a pioneer plant species with potential for stabilizing sand dunes and ecological restoration. It can settle on moving sand dunes earlier than other desert plants. It can effectively improve the stability of sand dunes and help more plants settle down and increase plant diversity. However, despite its important ecological value, the genetic resources available for this species are limited.ResultsWe used single-molecule real-time sequencing technology to obtain the complete full-length transcriptome ofStipagrostis pennata, including 90,204 unigenes with an average length of 2624 bp. In addition, the 5436 transcription factors identified in these unigenes are rich in stress resistance genes, such as MYB-related, C3H, bHLH, GRAS and HSF,etc., which may play a role in adapting to desert drought and strong wind stress. Intron retention events are abundant alternative splicing events. Stipagrostis pennatahas experienced stronger positive selection, accelerating the fixation of advantageous variants. Thirty-eight genes, such as CPP/TSO1-like gene, have evolved rapidly and may play a role in material transportation, flowering and seed formation.ConclusionsThe present study captures the complete full-length transcriptome ofStipagrostis pennataand reveals its rapid evolution. The desert adaptation inStipagrostis pennatais reflected in the regulation of gene expression and the adaptability of gene function. Our findings provide a wealth of knowledge for the evolutionary adaptability of desert grass species.
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影响因子：
14.9
作者：
Kanehisa M;Furumichi M;Sato Y;Ishiguro-Watanabe M;Tanabe M
通讯作者：
Tanabe M
影响因子：
3.7
作者：
Shi Y;Yan X;Zhao P;Yin H;Zhao X;Xiao H;Li X;Chen G;Ma XF
通讯作者：
Ma XF
AStrap：在没有参考基因组的情况下从转录序列中鉴定选择性剪接
影响因子：
5.8
作者：
Ji, Guoli;Ye, Wenbin;Wu, Xiaohui
通讯作者：
Wu, Xiaohui
影响因子：
11.6
作者：
Gomez, Elisa;Royo, Joaquin;Hueros, Gregorio
通讯作者：
Hueros, Gregorio
DOI：
10.4137/ebo.s22067
发表时间：
2015
期刊：
Evolutionary bioinformatics online
影响因子：
--
作者：
Ma J;Lu J;Xu J;Duan B;He X;Liu J
通讯作者：
Liu J