Temporal expression of Laminin-111 in the developing rat larynx.
Temporal expression of Laminin-111 in the developing rat larynx.
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DOI：
10.1016/j.neulet.2022.136658
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发表时间：
2022-06-11
影响因子：
2.5
通讯作者：
Pitman, Michael J.
中科院分区：
文献类型：
--
作者：
Caplan, Ian F.;Hernandez-Morato, Ignacio;Pitman, Michael J.
层粘连蛋白-111是一种参与运动神经支配和神经再支配的基底膜蛋白。在轴突寻路过程中，层粘连蛋白-111与netrin-1（NTN 1）相互作用，并将其吸引生长锥特性变为排斥。虽然先前的喉返神经（RLN）横断模型显示损伤后层粘连蛋白-111和NTN 1的产生增加，但尚未确定喉中的发育表达。本研究探讨了层粘连蛋白-111在喉肌中的表达，在喉初级神经支配的Sprague道利大鼠。将成人喉和胚胎切片，用β III-微管蛋白、层粘连蛋白亚基α-1（LAMA 1）、NTN 1和α-银环蛇毒素进行免疫组化。对切片进行LAMA 1 mRNA的单分子廉价RNA荧光原位杂交分析。LAMA 1表达增加，在所有喉内肌，除了内侧甲杓（MTA），在E20.5。在E20.5，与MTA相比，甲杓外侧（LTA）和环杓后（PCA）的表达增加。在E16.5时，NTN 1的上调仅限于LTA和环杓外侧肌（LCA），而MTA或PCA没有任何增加。LAMA 1和NTN 1的表达并没有严格遵循预期的模式相对于已知的神经支配的时间，并没有出现类似的作用后，RLN损伤。发育和损伤后神经支配之间的这些差异为神经损伤后的治疗研究提供了目标。
Laminin-111 is a basement membrane protein that participates in motor innervation and reinnervation. During axonal pathfinding, laminin-111 interacts with netrin-1 (NTN1) and changes its attractant growth cone properties into repulsion. While previous models of recurrent laryngeal nerve (RLN) transection show increased Laminin-111 and NTN1 production after injury, developmental expression in the larynx has not been defined. This study investigates the expression of laminin-111 in laryngeal muscles during primary laryngeal innervation of Sprague Dawley rats. Adult larynges and embryos were sectioned for immunohistochemistry with (βIII-Tubulin, laminin subunit α-1 (LAMA1), NTN1, and α-bungarotoxin. Sections were processed for single-molecule inexpensive RNA fluorescence in situ hybridization analysis of LAMA1 mRNA. LAMA1 expression increased in all intrinsic laryngeal muscles, except the medial thyroarytenoid (MTA), at E20.5. At E20.5 there was increased expression in the lateral thyroarytenoid (LTA) and posterior cricoarytenoid (PCA) compared to the MTA. NTN1 upregulation was limited to the LTA and lateral cricoarytenoid (LCA) at E16.5 without any increase in the MTA or PCA. LAMA1 and NTN1 expression did not strictly follow expected patterns relative to the known timing of innervation and does not appear to be acting similarly to its role following RLN injury. These differences between developmental and post-injury innervation provide targets for investigations of therapeutics after nerve injury.
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