CabZIP53 is targeted by CaWRKY40 and act as positive regulator in pepper defense against Ralstonia solanacearum and thermotolerance
CabZIP53 is targeted by CaWRKY40 and act as positive regulator in pepper defense against Ralstonia solanacearum and thermotolerance
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CabZIP53 是 CaWRKY40 的靶标，可作为辣椒防御青枯菌和耐热性的正调节因子
DOI：
10.1016/j.envexpbot.2018.12.017
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发表时间：
2019-03
影响因子：
5.7
通讯作者：
He Shuilin
中科院分区：
文献类型：
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作者：
Noman Ali;Hussain Ansar;Ashraf Muhammad Furqan;Khan Muhammad Ifnan;Liu Zhiqin;He Shuilin
病原体入侵后广泛的转录变化决定了对这些入侵者的免疫反应。辣椒植株对病原菌青枯菌和高温高湿条件的反应受不同转录因子的调控。然而，其基本的分子机制尚不清楚。不同的辣椒ZIP转录因子(TF)在植物防御中的功能还不清楚，尽管它们在响应生物胁迫中具有重要作用。在本研究中，我们从功能上鉴定了一个定位于bZIP Tf的核，CabZIP53参与了辣椒对青枯拉氏菌(RSI)和HTHH的防御。CabZIP53在辣椒植株中被RSI或HTHH转录诱导。通过病毒诱导的基因沉默(VIGS)进行的功能丧失研究表明，CabZIP53显著削弱了辣椒对RSI的基础防御，促进了病原菌的生长，降低了对HTHH的耐性，并抑制了免疫和耐热相关标记基因CaHIR1、CaPR1、CaNPR1、CaABR1、CabZIP63和CaHSP24的表达。同样，在农杆菌细胞抑制35S：：CabZIP53-Hain辣椒叶片的瞬时过表达诱导了类过敏性反应(HR)样的细胞死亡，H_2O_2的增加，并上调了免疫/热激反应相关的标记基因以及CaWRKY6 CaWRKY40，CabZIP63和CaCDPK15。QRT-PCR分析表明，CabZIP53与CaWRKY40形成正反馈环。同时，我们确定CabZIP53受CaWRKY40直接调控，但间接受CaWRKY6调控。总体而言，我们的结果表明，CabZIP53通过转录网络和涉及CaWRKY40的正反馈环来积极调节植物对RSI的免疫和对HTHH的耐性。
Broad range transcriptional changes after pathogen invasion decide immune responses to these invaders. The responses ofCapsicum annuumplants to bacterial pathogenRalstonia solanacearumand high-temperature-high-humidity (HTHH) conditions are coordinated by different transcription factors. However, the basic molecular mechanism is not clear. The functions of differentCapsicum annuumbZIP transcription factors (TFs) in plant defense are obscured in spite of their great importance in responses to biotic stress. In present study, we functionally characterized a nucleus localized bZIP TF,CabZIP53,for its involvement in pepper defense toRalstonia solanacearuminoculation (RSI) and HTHH.CabZIP53is transcriptionally induced in pepper plants by RSI or HTHH. Loss of function studies by Virus-induced gene silencing (VIGS) ofCabZIP53showed significantly impaired pepper basal defense against RSI, increased pathogen growth, decreased HTHH tolerance and hampered expression of immunity- and thermotolerance-associated marker genes such asCaHIR1,CaPR1, CaNPR1,CaABR1, CabZIP63andCaHSP24respectively. Similarly, transient overexpression ofCabZIP53mediatedbyAgrobacterium cells harbouring35S::CabZIP53-HAin pepper leaves induced hypersensitive response (HR)-like mimic cell death, H2O2accretion and up-regulated the immunity/thermotoelrance associated marker genes as well asCaWRKY6 CaWRKY40, CabZIP63andCaCDPK15. qRT-PCR analysis revealed that CabZIP53 makes a positive feed back loop withCaWRKY40. In parallel, we determined thatCabZIP53was regulated directly by CaWRKY40 but indirectly by CaWRKY6. On the whole, our results suggest thatCabZIP53positively regulates plant immunity to RSI and tolerance against HTHH by means of transcriptional networks and positive feedback loops involvingCaWRKY40.
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