Functional and Promoter Analysis of ChiIV3, a Chitinase of Pepper Plant, in Response to Phytophthora capsici Infection.
Functional and Promoter Analysis of ChiIV3, a Chitinase of Pepper Plant, in Response to Phytophthora capsici Infection.
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辣椒植物几丁质酶 ChiIV3 响应辣椒疫霉感染的功能和启动子分析
DOI：
10.3390/ijms18081661
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发表时间：
2017-08-01
影响因子：
5.6
通讯作者：
He S
中科院分区：
文献类型：
--
作者：
Liu Z;Shi L;Yang S;Lin Y;Weng Y;Li X;Hussain A;Noman A;He S
尽管几丁质酶家族的许多成员参与植物免疫，但大多数成员的确切功能仍然知之甚少。在此，发现辣椒中编码含有几丁质结合结构域并靶向质膜的几丁质酶蛋白的基因ChiIV 3通过辣椒疫霉接种（PCI）和施加几丁质处理诱导。除了对辣椒疫霉（Phytophthora capsici，P. capsici）生长的直接抑制作用外，通过辣椒植物中病毒诱导的基因沉默（VIGS）和瞬时过表达（TOE）也发现ChiIV 3作为植物细胞死亡的正调控因子，并触发防御信号和上调针对PCI的PR（发病相关）基因。5′端缺失分析显示pChiIV 3 −712至−459 bp足以使ChiIV 3 ′端对PCI产生应答。此外，突变测定表明pChiIV 3 −712至−459 bp中的W-box−466至−461 bp被注意到是PCI负责元件。这些结果共同表明，ChiIV 3作为一种可能的抗真菌蛋白，并作为植物中未知甲壳素的受体，触发细胞死亡和针对PCI的防御信号。
Despite the involvement of many members of the chitinase family in plant immunity, the precise functions of the majority of the members remain poorly understood. Herein, the gene ChiIV3 in Capsicum annuum encoding a chitinase protein containing a chitin binding domain and targeting to the plasma membrane was found to be induced by Phytophthora capsici inoculation (PCI) and applied chitin treatment. Besides its direct inhibitory effect on growth of Phytophthora capsici (P. capsici), ChiIV3 was also found by virus-induced gene silencing (VIGS) and transient overexpression (TOE) in pepper plants to act as a positive regulator of plant cell death and in triggering defense signaling and upregulation of PR (pathogenesis related) genes against PCI. A 5′ deletion assay revealed that pChiIV3−712 to −459 bp was found to be sufficient for ChiIV3’ response to PCI. Furthermore, a mutation assay indicated that W-box−466 to −461 bp in pChiIV3−712 to −459 bp was noted to be the PCI-responsible element. These results collectively suggest that ChiIV3 acts as a likely antifungal protein and as a receptor for unidentified chitin in planta to trigger cell death and defense signaling against PCI.
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影响因子：
11.4
作者：
Eulgem, T;Rushton, PJ;Somssich, IE
通讯作者：
Somssich, IE
影响因子：
6.9
作者：
Kim DS;Kim NH;Hwang BK
通讯作者：
Hwang BK
DOI：
10.1073/pnas.85.18.6662
发表时间：
1988-09-01
影响因子：
11.1
作者：
FINK, JS;VERHAVE, M;GOODMAN, RH
通讯作者：
GOODMAN, RH
影响因子：
6.5
作者：
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通讯作者：
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影响因子：
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作者：
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Hwang, BK