Compartmentalization of the somite and myogenesis in chick embryos are influenced by wnt expression.
Compartmentalization of the somite and myogenesis in chick embryos are influenced by wnt expression.
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鸡胚体节的区室化和肌发生受 wnt 表达的影响。
DOI：
10.1006/dbio.2000.9921
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发表时间：
2000
影响因子：
2.7
通讯作者：
B. Christ
中科院分区：
文献类型：
--
作者：
J. Wagner;C. Schmidt;W. Nikowits;B. Christ
身体的肌肉和中轴骨骼的骨骼来自体节的特殊区域。体节发育受邻近构造的影响。特别是，背侧神经管和覆盖的外胚层被证明是在皮肌节中诱导肌源性前体细胞所必需的。在背侧神经管和外胚层表达的Wnt信号分子家族成员被认为是负责这一过程的成员。这表明异位移植WNT-1、-3a和-4表达细胞改变了体内体节的区域化过程。在神经管/脊索和上皮性体节之间的腹侧植入了表达Wnt的细胞，导致背侧室扩大，损害了腹侧室--硬结。因此，异位Wnt的表达能够克服来自脊索和底板的腹壁信号的影响。在异位Wnt信号附近的体节中，Pax-3的表达区域增加，而Pax-1的表达完全丧失，证明了这两个隔室之间的边界的这种移位。MyoD和desmin的扩展表达提供了证据，表明Wnt信号导致的是肌节的增加。Wnt表达的细胞植入后，Paraxis的表达也显著增强，表明Wnts参与了体节上皮的形成和维持，提示Paraxis是通过Wnt信号通路激活的。综上所述，这些结果表明，异位的WNTs扰乱了躯体内信号分子的正常平衡，导致躯体细胞增加到肌源性谱系中。
Muscles of the body and bones of the axial skeleton derive from specialized regions of somites. Somite development is influenced by adjacent structures. In particular, the dorsal neural tube and the overlying ectoderm have been shown to be necessary for the induction of myogenic precursor cells in the dermomyotome. Members of the Wnt family of signaling molecules, which are expressed in the dorsal neural tube and the ectoderm, are postulated to be responsible for this process. It is shown here that ectopically implanted Wnt-1-, -3a-, and -4-expressing cells alter the process of somite compartmentalization in vivo. An enlarged dorsal compartment results from the implantation of Wnt-expressing cells ventrally between the neural tube/notochord and epithelial somites, at the expense of the ventral compartment, the sclerotome. Thus, ectopic Wnt expression is able to override the influence of ventralizing signals arising from notochord and floor plate. This shift of the border between the two compartments was identified by an increase in the domain of Pax-3 expression and a complete loss of Pax-1 expression in somites close to the ectopic Wnt signal. The expanded expression of MyoD and desmin provides evidence that it is the myotome which increases as a result of Wnt signaling. Paraxis expression is also drastically amplified after implantation of Wnt-expressing cells indicating that Wnts are involved in the formation and maintenance of somite epithelium and suggesting that Paraxis is activated through Wnt signaling pathways. Taken together these results suggest that ectopic Wnts disturb the normal balance of signaling molecules within the somite, resulting in an enhanced recruitment of somitic cells into the myogenic lineage.
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通过外胚层和神经管衍生信号调节旁轴表达和体节形成。
DOI：
10.1006/dbio.1997.8561
发表时间：
1997
期刊：
Developmental biology.
影响因子：
--
作者：
Sosic,D;Brand-Saberi,B;Schmidt,C;Christ,B;Olson,EN
通讯作者：
Olson,EN
影响因子：
4.6
作者：
H. M. Stern;A. Brown;S. Hauschka
通讯作者：
H. M. Stern;A. Brown;S. Hauschka
影响因子：
4.6
作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
通讯作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
体节的生肌规范：轴向结构的诱导。
DOI：
10.1242/dev.120.6.1443
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Buffinger,N;Stockdale,FE
通讯作者：
Stockdale,FE
影响因子：
10.5
作者：
Reshef, R;Maroto, M;Lassar, AB
通讯作者：
Lassar, AB