Blood meal acquisition enhances arbovirus replication in mosquitoes through activation of the GABAergic system.
Blood meal acquisition enhances arbovirus replication in mosquitoes through activation of the GABAergic system.
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DOI：
10.1038/s41467-017-01244-6
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发表时间：
2017-11-02
影响因子：
16.6
通讯作者：
Cheng G
中科院分区：
文献类型：
--
作者：
Zhu Y;Zhang R;Zhang B;Zhao T;Wang P;Liang G;Cheng G
蚊子是一种食血昆虫，携带并传播许多人类病毒。然而，关于蚊子感染这些病毒的共同机制的信息很少。在这项研究中，我们揭示了蚊子的嗜血性导致了血餐后的虫媒病毒感染，这种感染通过激活GABA能途径来抑制抗病毒的先天免疫。DsRNA介导的GABA信号的阻断和GABAA受体的阻断都显著地削弱了虫媒病毒的复制。一致地，接种GABA增强了虫媒病毒的感染，表明GABA信号有助于蚊子的虫媒病毒感染。蚊子对血液的摄取导致血液蛋白质消化产生的谷氨酸产生强劲的GABA。口服谷氨酸通过激活GABA能系统增加了蚊子获取病毒的能力。我们的研究表明，血粉通过激活GABA能系统增强蚊子中虫媒病毒的复制。许多人类病毒的传播依赖于蚊子媒介的复制。在这里，朱等人。研究表明，从血餐中消化的谷氨酸可以激活GABA信号，从而抑制蚊子体内的抗病毒天然免疫并增加病毒复制。
Mosquitoes are hematophagous insects that carry-on and transmit many human viruses. However, little information is available regarding the common mechanisms underlying the infection of mosquitoes by these viruses. In this study, we reveal that the hematophagous nature of mosquitoes contributes to arboviral infection after a blood meal, which suppresses antiviral innate immunity by activating the GABAergic pathway. dsRNA-mediated interruption of the GABA signaling and blockage of the GABAA receptor by the specific inhibitors both significantly impaired arbovirus replication. Consistently, inoculation of GABA enhanced arboviral infection, indicating that GABA signaling facilitates the arboviral infection of mosquitoes. The ingestion of blood by mosquitoes resulted in robust GABA production from glutamic acid derived from blood protein digestion. The oral introduction of glutamic acid increased virus acquisition by mosquitoes via activation of the GABAergic system. Our study reveals that blood meals enhance arbovirus replication in mosquitoes through activation of the GABAergic system. Transmission of many human viruses depends on replication in their mosquito vectors. Here, Zhu et al. show that glutamic acid digested from the blood meal activates GABA signaling, resulting in suppression of antiviral innate immunity and increased virus replication in mosquitoes.
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DOI：
10.3390/v7020820
发表时间：
2015-02-17
期刊：
Viruses
影响因子：
--
作者：
Blair CD;Olson KE
通讯作者：
Olson KE
影响因子：
3.3
作者：
Jupin, Marc;Michiels, Paul J.;Wijmenga, Sybren S.
通讯作者：
Wijmenga, Sybren S.
影响因子：
2.5
作者：
Kinel-Tahan, Yael;Weiss, Hanna;Wides, Ron
通讯作者：
Wides, Ron
DOI：
10.1073/pnas.1412984112
发表时间：
2015-01-13
影响因子：
11.1
作者：
Carissimo, Guillaume;Pondeville, Emilie;Vernick, Kenneth D.
通讯作者：
Vernick, Kenneth D.
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC