Automated semen analysis by SQA Vision® versus the manual approach—A prospective double‐blind study
Automated semen analysis by SQA Vision® versus the manual approach—A prospective double‐blind study
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SQA Vision® 自动精液分析与手动方法的对比——一项前瞻性双盲研究
作者：
Engel KM;Grunewald S;Schiller J;Paasch U
除了临床研究外，男性生育力的评估主要依赖于详细的精子分析，例如细胞计数、运动性、细胞形态和活力测试。手动创建精子图既耗时又耗材。因此，迫切需要可替代手动方法的可靠的高通量系统。本研究旨在比较按照WHO第5版指南进行的常规精子分析和使用SQA Vision®机器进行的精液分析。SQA Vision®是一种用于自动精子分析的商用设备。采用Bland-Altman图和Passing-Bablok回归分析，通过统计分析比较了两种方法独立获得的数据。分析显示，精子浓度和总活力的结果相当。前向运动力的一致性较差，正常精子形态的测定存在明显偏差。Passing-Bablok回归分析和95%置信区间的考虑指出了手动精液分析和自动方法之间的系统性和比例差异。
Next to clinical investigations, the evaluation of male fertility relies mainly on detailed sperm analyses, for example, cell counting, motility, cell morphology and vitality testing. The manual creation of a spermiogram is time‐ and material‐consuming. Therefore, reliable high‐throughput systems that may be substituted for manual methods are urgently needed. The present study aimed to compare conventional sperm analysis performed as per WHO 5th guidelines and semen analysis performed with the SQA Vision®machine. SQA Vision®is a commercial device for automated sperm analysis. Data obtained independently by both methods were compared by statistical analyses using Bland–Altman plots and Passing–Bablok regression analyses. The analyses revealed that the results for sperm concentration and total motility were comparable. The agreement for progressive motility was poor, and there were clear deviations in the determination of normal sperm morphology. Passing–Bablok regression analyses and the consideration of the 95% confidence intervals pointed out systematic and proportional differences between the manual semen analysis and the automated approach.
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