Metabolic engineering for the production of natural products.
Metabolic engineering for the production of natural products.
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DOI：
10.1146/annurev-chembioeng-061010-114209
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发表时间：
2011
影响因子：
8.4
通讯作者：
Chooi YH
中科院分区：
文献类型：
--
作者：
Pickens LB;Tang Y;Chooi YH
天然产物和天然产物衍生化合物在现代医疗保健中发挥着重要作用，作为许多疾病的一线治疗方法和化学合成疗法的灵感。随着测序和重组 DNA 技术的进步，许多负责生产这些化学复杂且具有药学价值的化合物的生物合成途径已被阐明。随着生物合成成分工具包的不断扩展，代谢工程成为提高天然产物效价和生成新型化合物的日益强大的方法。异源生产平台使得能够获得从难以培养的菌株中获得的途径；系统生物学和代谢建模工具提高了预测和分析能力；表达系统和调控的进步使得能够微调途径以提高效率，并且各个途径成分的表征促进了用于生产新化合物的混合途径的构建。代谢工程在许多方面的进步不仅产生了令人着迷的科学发现，而且使其成为优化天然产物生物合成日益可行的方法。
Natural products and natural product derived compounds play an important role in modern healthcare as frontline treatments for many diseases and as inspiration for chemically synthesized therapeutics. With advances in sequencing and recombinant DNA technology, many of the biosynthetic pathways responsible for the production of these chemically complex and pharmaceutically valuable compounds have been elucidated. With an ever expanding toolkit of biosynthetic components, metabolic engineering is an increasingly powerful method to improve natural product titers and generate novel compounds. Heterologous production platforms have enabled access to pathways from difficult to culture strains; systems biology and metabolic modeling tools have resulted in increasing predictive and analytic capabilities; advances in expression systems and regulation have enabled the fine-tuning of pathways for increased efficiency, and characterization of individual pathway components has facilitated the construction of hybrid pathways for the production of new compounds. These advances in the many aspects of metabolic engineering have not only yielded fascinating scientific discoveries but also make it an increasingly viable approach for the optimization of natural product biosynthesis.
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影响因子：
5.1
作者：
Eustaquio, Alessandra S.;O'Hagan, David;Moore, Bradley S.
通讯作者：
Moore, Bradley S.
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
3.2
作者：
Chen, Yihua;Wendt-Pienkowski, Evelyn;Shen, Ben
通讯作者：
Shen, Ben
DOI：
10.1073/pnas.1934677100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Challis, GL;Hopwood, DA
通讯作者：
Hopwood, DA
影响因子：
3.4
作者：
Cropp, A;Chen, S;Reynolds, KA
通讯作者：
Reynolds, KA