Bacterial microbiome and host inflammatory gene expression in foreskin tissue.
Bacterial microbiome and host inflammatory gene expression in foreskin tissue.
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细菌微生物组和包皮组织中的炎症基因表达。
DOI：
10.1016/j.heliyon.2023.e22145
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发表时间：
2023-11
期刊：
影响因子：
4
通讯作者：
Jaspan, Heather B.
中科院分区：
文献类型：
--
作者：
Maust, Brandon S.;Petkov, Stefan;Herrera, Carolina;Feng, Colin;Brown, Bryan P.;Lebina, Limakatso;Opoka, Daniel;Ssemata, Andrew;Pillay, Natasha;Serwanga, Jennifer;Seatlholo, Portia;Namubiru, Patricia;Odoch, Geoffrey;Mugaba, Susan;Seiphetlo, Thabiso;Gray, Clive M.;Kaleebu, Pontiano;Webb, Emily L.;Martinson, Neil;Chiodi, Francesca;Fox, Julie;Jaspan, Heather B.
阴茎上皮微生物组仍然未被探索。我们对来自南非和乌干达的144名青少年在接受1-2剂安慰剂、恩曲他滨+富马酸替诺福韦酯或恩曲他滨+替诺福韦艾拉酚胺后进行阴茎包皮环切术期间收集的包皮组织中的人类RNA和细菌16 S rRNA基因片段进行测序，以研究包皮组织的微生物组及其抗逆转录病毒药物使用的潜在变化。我们发现了大量的厌氧物种，包括痤疮棒状杆菌，这是更频繁地检测到来自南非的参与者比乌干达。细菌种群没有不同的治疗接受，并没有差异丰富的类群之间的安慰剂与活性药物接受者。特定细菌分类群的相对丰度与对细菌的先天免疫应答下游基因的表达和炎症调节呈负相关。我们的研究结果表明，短期使用抗逆转录病毒药物的包皮组织微生物组没有差异，但细菌分类群在很大程度上与炎性基因表达呈负相关，这与包皮定植一致。
The penile epithelial microbiome remains underexplored. We sequenced human RNA and a segment of the bacterial 16S rRNA gene from the foreskin tissue of 144 adolescents from South Africa and Uganda collected during penile circumcision after receipt of 1–2 doses of placebo, emtricitabine + tenofovir disoproxil fumarate, or emtricitabine + tenofovir alafenamide to investigate the microbiome of foreskin tissue and its potential changes with antiretroviral use. We identified a large number of anaerobic species, including Corynebacterium acnes, which was detected more frequently in participants from South Africa than Uganda. Bacterial populations did not differ by treatment received, and no differentially abundant taxa were identified between placebo versus active drug recipients. The relative abundance of specific bacterial taxa was negatively correlated with expression of genes downstream of the innate immune response to bacteria and regulation of inflammation. Our results show no difference in the tissue microbiome of the foreskin with short-course antiretroviral use but that bacterial taxa were largely inversely correlated with inflammatory gene expression, consistent with commensal colonization.
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