Characterization of LRL5 as a key regulator of root hair growth in maize
Characterization of LRL5 as a key regulator of root hair growth in maize
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LRL5 作为玉米根毛生长关键调节因子的表征
DOI：
10.1111/tpj.14200
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Chen Li-Qun
中科院分区：
文献类型：
--
作者：
Wang Chun-Xia;Qi Chuang-Ye;Luo Jin-Hong;Liu Lin;He Yan;Chen Li-Qun
根毛是一种特殊的管状细胞，从根表皮细胞中长出，在植物吸收养分和水分以及与生物和非生物胁迫的相互作用中起着重要作用。对模式植物拟南芥的研究表明，根毛的起始和伸长受到一组碱性螺旋-环-螺旋（bHLH）转录因子（TF）的分级调节。然而，关于这些bHLH TF在控制根毛生长中的调节途径的知识仍然有限。在这项研究中，进行了RNA-seq分析以分析伸长玉米根毛中的转录组，并鉴定了>1000个在根毛中优先表达的基因。一个共同的顺式元件以前的功能作为潜在的bHLH-TF结合位点存在于大多数的根毛优先表达基因的调控区。此外，玉米根毛中表达最高的bHLH-TF ZmLRL 5的个体变化导致根毛伸长的显著减少，并使根毛的生长对翻译抑制高度敏感。此外，RNA-seq，酵母单杂交和核糖体谱分析表明，ZmLRL 5可能作为一个关键球员在协调翻译过程中，通过直接调节翻译过程/核糖体基因的表达在玉米根毛生长。
Root hair, a special type of tubular-shaped cell, outgrows from the root epidermal cell and plays important roles in the acquisition of nutrients and water, as well as interactions with biotic and abiotic stresses. Studies in the model plant Arabidopsis have revealed that root-hair initiation and elongation are hierarchically regulated by a group of basic helix-loop-helix (bHLH) transcription factors (TFs). However, knowledge regarding the regulatory pathways of these bHLH TFs in controlling root hair growth remains limited. In this study, RNA-seq analysis was conducted to profile the transcriptome in the elongating maize root hair and >1000 genes with preferential expression in root hair were identified. A consensus cis-element previously featured as the potential bHLH-TF binding sites was present in the regulatory regions for the majority of the root hair-preferentially expressed genes. In addition, an individual change in ZmLRL5, the highest-expressed bHLH-TF in maize root hair resulted in a dramatic reduction in the elongation of root hair, and rendered the growth of root hair hypersensitive to translational inhibition. Moreover, RNA-seq, yeast-one-hybrid and ribosome profile analysis suggested that ZmLRL5 may function as a key player in orchestrating the translational process by directly regulating the expression of translational processes/ribosomal genes during maize root hair growth.
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DOI：
10.1111/nph.14355
发表时间：
2017-04
期刊：
The New phytologist
影响因子：
--
作者：
Bai B;Peviani A;van der Horst S;Gamm M;Snel B;Bentsink L;Hanson J
通讯作者：
Hanson J
eIF5B 突变通过翻译缺陷赋予拟南芥热敏性和多效性表型
影响因子：
11.6
作者：
Zhang Liyuan;Liu Xinye;Gaikwad Kishor;Kou Xiaoxia;Wang Fei;Tian Xuejun;Xin Mingming;Ni Zhongfu;Sun Qixin;Peng Huiru;Vierling Elizabeth
通讯作者：
Vierling Elizabeth
agriGO v2.0：农业社区的 GO 分析工具包，2017 年更新
影响因子：
14.9
作者：
Tian T;Liu Y;Yan H;You Q;Yi X;Du Z;Xu W;Su Z
通讯作者：
Su Z
影响因子：
7.2
作者：
Hetz, W;Hochholdinger, F;Feix, G
通讯作者：
Feix, G
影响因子：
4.5
作者：
Bruex A;Kainkaryam RM;Wieckowski Y;Kang YH;Bernhardt C;Xia Y;Zheng X;Wang JY;Lee MM;Benfey P;Woolf PJ;Schiefelbein J
通讯作者：
Schiefelbein J