Effect of the biosynthesis of the volatile compound phenylacetaldehyde on chloroplast modifications in tea (Camellia sinensis) plants.
Effect of the biosynthesis of the volatile compound phenylacetaldehyde on chloroplast modifications in tea (Camellia sinensis) plants.
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挥发性化合物苯乙醛的生物合成对茶树叶绿体修饰的影响
DOI：
10.1093/hr/uhad003
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发表时间：
2023-03
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
摘要 植物挥发性化合物具有重要的生理和生态功能。苯乙醛 (PAld) 是一种挥发性苯丙类化合物/苯类化合物，在连续遮荫下生长的茶树 (Camellia sinensis) 叶子中积累。本研究旨在确定茶树中 PAld 的产生是否与光相关，并阐明 PAld 的生理功能。具体而言，研究了不同光照条件下调节茶树PAld生物合成的上游机制以及PAld对叶绿体/叶绿素的影响。 PAld 的生物合成在光照下受到抑制，而在黑暗下则被诱导。编码芳香族氨基酸转氨酶 1 (CsAAAT1) 的结构基因在黑暗中高水平表达，这与其对 PAld 积累的重要性一致。此外，转录激活测定和电泳迁移率变动测定的结果表明，CsAAAT1 表达被光敏色素相互作用因子 3-2 (CsPIF3-2)（一种光响应转录因子）轻微激活。此外，PAld 可能会促进暗处理叶绿体中叶绿素的激发，并介导细胞中的电子能量转移。然而，积累的 PAld 会降解叶绿体和叶绿素，对光合作用产生潜在的有害影响。此外，红光和蓝光会抑制茶叶中PAld的生物合成，从而减少白天PAld对叶绿体的不利影响。总之，茶树在光照和黑暗条件下调节 PAld 生物合成会导致叶绿体发生改变。这项研究的结果扩大了我们对茶叶中挥发性苯丙素/苯类化合物的生物合成和功能的理解。
Abstract Plant volatile compounds have important physiological and ecological functions. Phenylacetaldehyde (PAld), a volatile phenylpropanoid/benzenoid, accumulates in the leaves of tea (Camellia sinensis) plants grown under continuous shading. This study was conducted to determine whether PAld production is correlated with light and to elucidate the physiological functions of PAld in tea plants. Specifically, the upstream mechanism modulating PAld biosynthesis in tea plants under different light conditions as well as the effects of PAld on chloroplast/chlorophyll were investigated. The biosynthesis of PAld was inhibited under light, whereas it was induced in darkness. The structural gene encoding aromatic amino acid aminotransferase 1 (CsAAAT1) was expressed at a high level in darkness, consistent with its importance for PAld accumulation. Additionally, the results of a transcriptional activation assay and an electrophoretic mobility shift assay indicated CsAAAT1 expression was slightly activated by phytochrome-interacting factor 3-2 (CsPIF3-2), which is a light-responsive transcription factor. Furthermore, PAld might promote the excitation of chlorophyll in dark-treated chloroplasts and mediate electron energy transfer in cells. However, the accumulated PAld can degrade chloroplasts and chlorophyll, with potentially detrimental effects on photosynthesis. Moreover, PAld biosynthesis is inhibited in tea leaves by red and blue light, thereby decreasing the adverse effects of PAld on chloroplasts during daytime. In conclusion, the regulated biosynthesis of PAld in tea plants under light and in darkness leads to chloroplast modifications. The results of this study have expanded our understanding of the biosynthesis and functions of volatile phenylpropanoids/benzenoids in tea leaves.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.6
作者：
Fu X;Chen Y;Mei X;Katsuno T;Kobayashi E;Dong F;Watanabe N;Yang Z
通讯作者：
Yang Z
影响因子：
4.2
作者：
Gil, Mariana;Bottini, Ruben;Piccoli, Patricia
通讯作者：
Piccoli, Patricia
影响因子：
56.9
作者：
Martínez-García, JF;Huq, E;Quail, PH
通讯作者：
Quail, PH
影响因子：
3.5
作者：
Kilpatrick, Korey;Pajak, Agnieszka;Marsolais, Frederic
通讯作者：
Marsolais, Frederic