Killing of Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia by protegrins.
Killing of Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia by protegrins.
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protegrins 杀死具核梭杆菌、牙龈卟啉单胞菌和中间普氏菌。
DOI：
10.1111/j.1600-0765.1998.tb02297.x
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发表时间：
1998
影响因子：
3.5
通讯作者：
Lehrer,RI
中科院分区：
文献类型：
--
作者：
Miyasaki,KT;Iofel,R;Oren,A;Huynh,T;Lehrer,RI
Protegrins是从猪白细胞中分离的广谱抗生素肽。在这项研究中，我们（i）检查革兰氏阴性厌氧牙周病原体对合成保护素的敏感性;（ii）确定保护素同源物对这些细菌的相对效力;（iii）比较保护素与其他抗生素肽的效力，包括magainin MSI-78，tachyplesin I，cecropin P1，人类防御素HNP-1 - 3和clavanin A。对合成的保护蛋白1的l-和d-对映体（分别为PG-1和D-PG-1）以及保护蛋白2、3和5的L-对映体（PG-2、PG-3和PG-5）进行了抗具核梭杆菌和黑色色素生物体（包括牙龈卟啉单胞菌和中间普雷沃氏菌）的测试。核瘤和黑色色素微生物对PG-1敏感，平均ED 99分别为2.2 - 2.3 μg/ml和3.4 - 9.9 μg/ml。D型在统计学上比L型对这些口腔厌氧菌更有效，尽管这种效力差异不太可能具有决定性的治疗意义，但鉴于其抵抗微生物和宿主来源的蛋白酶的能力，D型可能具有价值。在测试条件下，PG-1比爪蟾抗菌肽、鲎素、天蚕抗菌肽、防御素和克拉维宁更有效。发现高渗盐浓度和热灭活血清对PG-1的杀菌活性具有抑制作用。发现PG-1诱导F的超微结构外观的形态学改变。与细菌细胞膜损伤一致。我们的结论是，protegrins可能是有用的抗菌剂，在治疗革兰氏阴性厌氧菌被认为是参与慢性，成人形式的牙周感染。
Protegrins are broad spectrum antibiotic peptides isolated from porcine leukocytes. In this study, we (i) examine the sensitivity of Gram‐negative, anaerobic periodontal pathogens to synthetic protegrins; (ii) determine the relative potencies of protegrin congeners against these bacteria; and (iii) compare the potency of protegrins with other antibiotic peptides, including magainin MSI‐78, tachyplesin I, cecropin P1, human defensins HNP‐1‐3, and clavanin A. Syntheticl‐ andd‐enantiomers of protegrin 1 (PG‐1 and D‐PG‐1, respectively), and L‐enantiomers of protegrins 2, 3 and 5 (PG‐2, PG‐3 and PG‐5) were tested againstFusobacteriurn nucleatum, and black‐pigmented organisms includingPorphyromonas gingivalisandPrevotella intermedia.Strains of bothF. nucleatumand the black‐pigmented organisms were sensitive to PG‐1, and exhibited mean ED99of 2.2‐2.3 μg/ml and 3.4‐9.9 μg/ml, respectively. The D‐form was statistically more potent than the L‐form against these oral anaerobes, and although this difference in potency is unlikely to be of decisive therapeutic significance, thed‐form may be of value given ability to resist microbial and host‐derived proteases. PG‐1 was more potent than magainin, tachyplesin, cecropin, defensins and clavanin under test conditions. Hypertonic saIt concentrations and heat‐inactivated serum were found to be inhibitory to the bactericidal activity of PG‐1. PG‐1 was found to induce morphologic alterations in the ultrastructural appearance ofF. nucleatumconsistent with damage to the bacterial membranes. We conclude that protegrins may be useful antimicrobial agents in therapy against Gram‐negative anaerobic bacteria believed to be involved in chronic, adult forms of periodontal infections.
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裂隙液：来源、成分、收集方法和临床意义。
DOI：
--
发表时间：
1977
期刊：
影响因子：
--
作者：
Abbott Bh;Caffesse Rg
通讯作者：
Caffesse Rg
胰岛素依赖型糖尿病控制不佳的健康和患病牙周部位的微生物学。
影响因子：
4.3
作者：
Mandell,RL;Dirienzo,J;Kent,R;Joshipura,K;Haber,J
通讯作者：
Haber,J
影响因子：
15.9
作者：
LEHRER, RI;BARTON, A;SELSTED, ME
通讯作者：
SELSTED, ME
影响因子：
18.6
作者：
Moore, W E;Moore, L V
通讯作者：
Moore, L V
影响因子：
56.9
作者：
FIELDS, PI;GROISMAN, EA;HEFFRON, F
通讯作者：
HEFFRON, F