A Self-Referenced Diffraction-Based Optical Leaky Waveguide Biosensor Using Photofunctionalised Hydrogels.
A Self-Referenced Diffraction-Based Optical Leaky Waveguide Biosensor Using Photofunctionalised Hydrogels.
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DOI：
10.3390/bios10100134
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发表时间：
2020-09-24
期刊：
影响因子：
--
通讯作者：
Gupta R
中科院分区：
文献类型：
--
作者：
Pal AK;Goddard NJ;Dixon HJ;Gupta R
我们报道了一种新型的基于自参考衍射基的泄漏波导(LW)，它包含一层薄(~2微米)的光功能化水凝胶薄膜，该水凝胶是通过将生物素化的光可裂解连接物共价连接到壳聚糖上而形成的。通过光可裂解连接物将链霉亲和素结合到壳聚糖上，通过365 nm的光通过光掩模选择性地去除，以产生具有高和低蛋白质负载量的条带阵列，分别作为传感器和参照区。传感器和参照区之间的差异测量用于测量分析物(即生物素蛋白A和免疫球蛋白G)，同时减少环境和非特异性影响。这些影响包括非吸附物种和吸附物种引起的温度和样品组成的变化，导致影响比绝对测量分别降低约98%、~99%和~97%。这项工作的创新之处在于将光功能化水凝胶与基于衍射基的LWs相结合以供参考。这是实现无标签光学生物传感器测量复杂混合物的真实样品中分析物浓度的全部潜力所必需的，并允许在观察到温度漂移和波动的实验室外进行样品分析。
We report a novel self-referenced diffraction-based leaky waveguide (LW) comprising a thin (~2 µm) film of a photofunctionalisable hydrogel created by covalent attachment of a biotinylated photocleavable linker to chitosan. Streptavidin attached to the chitosan via the photocleavable linker was selectively removed by shining 365 nm light through a photomask to create an array of strips with high and low loading of the protein, which served as sensor and reference regions respectively. The differential measurements between sensor and reference regions were used for measuring analytes (i.e., biotin protein A and IgG) while reducing environmental and non-specific effects. These include changes in temperature and sample composition caused by non-adsorbing and adsorbing species, leading to reduction in effects by ~98%, ~99%, and ~97% respectively compared to the absolute measurements. The novelty of this work lies in combining photofunctionalisable hydrogels with diffraction-based LWs for referencing. This is needed to realise the full potential of label-free optical biosensors to measure analyte concentrations in real samples that are complex mixtures, and to allow for sample analysis outside of laboratories where drifts and fluctuations in temperature are observed.
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影响因子：
4.6
作者：
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