Levels of plasma glycan-binding auto-IgG biomarkers improve the accuracy of prostate cancer diagnosis.
Levels of plasma glycan-binding auto-IgG biomarkers improve the accuracy of prostate cancer diagnosis.
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DOI：
10.1007/s11010-020-03876-7
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发表时间：
2021-01
影响因子：
4.3
通讯作者：
Kim H
中科院分区：
文献类型：
--
作者：
Dos Santos JM;Joiakim A;Kaplan DJ;Putt DA;Perez Bakovic G;Servoss SL;Rybicki BA;Dombkowski AA;Kim H
提高前列腺癌早期诊断的策略将为早期干预提供机会。基于血液的前列腺特异性抗原（PSA）检测被广泛用于前列腺癌的诊断，但该检测的特异性并不令人满意。一种基于血清PSA水平结合其他血清生物标志物的算法可能显著提高前列腺癌的诊断。血浆聚糖结合IgG/IgM研究表明正常细胞和肿瘤细胞之间的聚糖模式不同。我们假设前列腺癌的糖蛋白或糖脂从肿瘤组织分泌到血液中并诱导自身免疫球蛋白（Ig）的产生。利用35名前列腺癌患者和54名健康受试者的血浆样本，建立了24糖基微阵列和5糖基亚阵列，以鉴定糖基结合的自体igg。前列腺癌样本中Neu5Acα2-8Neu5Acα2-8Neu5Acα (G81)结合的自身igg水平较高，当G81结合的自身igg水平和生长分化因子-15 （GDF-15或NAG-1）水平联合PSA水平时，前列腺癌的预测率从78.2%提高到86.2%。采用G81聚糖亲和层析法从血浆样品中分离G81聚糖结合的IgG片段，并对IgG的50 kDa重链可变区进行n端测序鉴定。通过n端测序还鉴定出G81聚糖结合25kda成纤维细胞生长因子-1 （FGF1）片段。我们的研究结果表明，结合G81聚糖结合自身igg、GDF-15/NAG-1和PSA（≥2.1 ng PSA/ml）的多重诊断可将前列腺癌的诊断特异性提高8%。多重评估可以提高前列腺癌的早期诊断，从而允许前列腺癌治疗的及时交付。
Strategies to improve the early diagnosis of prostate cancer will provide opportunities for earlier intervention. The blood-based prostate-specific antigen (PSA) assay is widely used for prostate cancer diagnosis but specificity of the assay is not satisfactory. An algorithm based on serum levels of PSA combined with other serum biomarkers may significantly improve prostate cancer diagnosis. Plasma glycan-binding IgG/IgM studies suggested that glycan patterns differ between normal and tumor cells. We hypothesize that in prostate cancer glycoproteins or glycolipids are secreted from tumor tissues into the blood and induce auto-immunoglobulin (Ig) production. A 24-glycan microarray and a 5-glycan subarray were developed using plasma samples obtained from 35 prostate cancer patients and 54 healthy subjects in order to identify glycan-binding auto-IgGs. Neu5Acα2–8Neu5Acα2–8Neu5Acα (G81)-binding auto-IgG was higher in prostate cancer samples and, when levels of G81-binding auto-IgG and growth differentiation factor-15 (GDF-15 or NAG-1) were combined with levels of PSA, the prediction rate of prostate cancer increased from 78.2% to 86.2% than with PSA levels alone. The G81 glycan-binding auto-IgG fraction was isolated from plasma samples using G81 glycan-affinity chromatography and identified by N-terminal sequencing of the 50 kDa heavy chain variable region of the IgG. G81 glycan-binding 25 kDa fibroblast growth factor-1 (FGF1) fragment was also identified by N-terminal sequencing. Our results demonstrated that a multiplex diagnostic combining G81 glycan-binding auto-IgG, GDF-15/NAG-1 and PSA (≥2.1 ng PSA/ml for cancer) increased the specificity of prostate cancer diagnosis by 8%. The multiplex assessment could improve the early diagnosis of prostate cancer thereby allowing the prompt delivery of prostate cancer treatment.
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影响因子：
2.8
作者：
Stephan, C;Xu, CL;Jung, K
通讯作者：
Jung, K
DOI：
10.3390/s17020256
发表时间：
2017-01-29
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Sauer U
通讯作者：
Sauer U
DOI：
10.1007/s00262-009-0733-4
发表时间：
2009-10
期刊：
Cancer immunology, immunotherapy : CII
影响因子：
--
作者：
Reuschenbach M;von Knebel Doeberitz M;Wentzensen N
通讯作者：
Wentzensen N
成纤维细胞生长因子 1 单链抗体的人源化及其对乳腺癌和神经胶质瘤细胞的抗肿瘤功效的验证。
影响因子：
5.3
作者：
He XX;Du S;Gao SQ;Chen JY;Cao RJ;Xing ZK;Kazim ARS;Yu HL;Zheng QC;Zhu XJ
通讯作者：
Zhu XJ
影响因子：
4.6
作者：
Totten SM;Adusumilli R;Kullolli M;Tanimoto C;Brooks JD;Mallick P;Pitteri SJ
通讯作者：
Pitteri SJ