Multi-lectin Affinity Chromatography and Quantitative Proteomic Analysis Reveal Differential Glycoform Levels between Prostate Cancer and Benign Prostatic Hyperplasia Sera.
Multi-lectin Affinity Chromatography and Quantitative Proteomic Analysis Reveal Differential Glycoform Levels between Prostate Cancer and Benign Prostatic Hyperplasia Sera.
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DOI：
10.1038/s41598-018-24270-w
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发表时间：
2018-04-25
影响因子：
4.6
通讯作者：
Pitteri SJ
中科院分区：
文献类型：
--
作者：
Totten SM;Adusumilli R;Kullolli M;Tanimoto C;Brooks JD;Mallick P;Pitteri SJ
目前前列腺特异性抗原用于前列腺癌（PCa）筛查，但缺乏区分PCa与良性前列腺增生（BPH）等其他前列腺疾病所需的特异性，临床需要在分子水平上区分这些病例。蛋白质糖基化在涉及肿瘤进展的许多细胞过程中发挥着重要作用，并且在前列腺癌中是异常的。在这项研究中，我们使用多凝集素亲和层析和基于定量质谱的蛋白质组学系统地研究了 PCa 和 BPH 样品中数百种血清蛋白及其糖型的循环水平的变化。使用特定的凝集素（AAL、PHA-L 和 PHA-E）来靶向并通过色谱分离核心岩藻糖基化和高度支化的蛋白质糖型进行分析，因为这些聚糖类型的差异表达先前已与 PCa 相关。 PCa 样本中 CD5L、CFP、C8A、BST1 和 C7 的整体水平显着升高。在 PHA-L/E 组分中的蛋白质 CD163、C4A 和 ATRN 以及 AAL 组分中的 C4BPB 和 AZGP1 糖型之间发现了 BPH 和 PCa 之间显着的糖型特异性改变。尽管存在这些微小的差异，但在 PCa 和 BPH 血清之间观察到糖蛋白组谱的显着相似性。
Currently prostate-specific antigen is used for prostate cancer (PCa) screening, however it lacks the necessary specificity for differentiating PCa from other diseases of the prostate such as benign prostatic hyperplasia (BPH), presenting a clinical need to distinguish these cases at the molecular level. Protein glycosylation plays an important role in a number of cellular processes involved in neoplastic progression and is aberrant in PCa. In this study, we systematically interrogate the alterations in the circulating levels of hundreds of serum proteins and their glycoforms in PCa and BPH samples using multi-lectin affinity chromatography and quantitative mass spectrometry-based proteomics. Specific lectins (AAL, PHA-L and PHA-E) were used to target and chromatographically separate core-fucosylated and highly-branched protein glycoforms for analysis, as differential expression of these glycan types have been previously associated with PCa. Global levels of CD5L, CFP, C8A, BST1, and C7 were significantly increased in the PCa samples. Notable glycoform-specific alterations between BPH and PCa were identified among proteins CD163, C4A, and ATRN in the PHA-L/E fraction and among C4BPB and AZGP1 glycoforms in the AAL fraction. Despite these modest differences, substantial similarities in glycoproteomic profiles were observed between PCa and BPH sera.
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影响因子：
5.8
作者：
Craig, R;Beavis, RC
通讯作者：
Beavis, RC
影响因子：
4.7
作者：
Korpetinou A;Skandalis SS;Labropoulou VT;Smirlaki G;Noulas A;Karamanos NK;Theocharis AD
通讯作者：
Theocharis AD
DOI：
10.1073/pnas.1013699108
发表时间：
2011-02-22
影响因子：
11.1
作者：
Cima, Igor;Schiess, Ralph;Krek, Wilhelm
通讯作者：
Krek, Wilhelm
影响因子：
4.8
作者：
De, S;Chen, JH;Byzova, TV
通讯作者：
Byzova, TV
影响因子：
--
作者：
Lanciotti M;Masieri L;Raspollini MR;Minervini A;Mari A;Comito G;Giannoni E;Carini M;Chiarugi P;Serni S
通讯作者：
Serni S