'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.
'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.
复制标题
DOI：
10.1111/1462-2920.16149
复制
发表时间：
2022-11
影响因子：
5.1
通讯作者：
Murrell, J. Colin
中科院分区：
文献类型：
--
作者：
Dawson, Robin A.;Rix, Gregory D.;Crombie, Andrew T.;Murrell, J. Colin
栖息在土壤和树叶中的细菌部分减轻了丰富的挥发性有机化合物异戊二烯（2-甲基-1，3-丁二烯）的释放。虽然20年前在红球菌属AD 45中鉴定了异戊二烯代谢的初始步骤，但异戊二烯代谢途径仍然在很大程度上不确定。对isoG、isoJ和aldH功能的有限理解以及异戊二烯衍生碳进入中枢代谢途径的不确定性阻碍了我们对异戊二烯代谢的理解。这些以前未表征的iso基因是必不可少的Variovorax sp. WS 11，通过靶向诱变确定。使用组合的基于组学的方法，我们提出了完整的异戊二烯代谢途径。异戊二烯转化为丙酰辅酶A，后者通过染色体编码的甲基丙二酰辅酶A途径同化，需要生物素和维生素B12，而质粒编码的柠檬酸甲酯途径可能提供针对这些维生素限制的稳健性。这一途径的关键组成部分，诱导异戊二烯和其初始氧化产物，环氧异戊二烯，异戊二烯代谢的主要诱导剂Variovorax的。WS 11和红球菌属。AD 45。不同异戊二烯降解细菌的基因组分析表明，甲基柠檬酸和甲基丙二酰辅酶A途径的所有遗传组分并不总是存在于异戊二烯降解菌中，尽管普遍表明通过丙酰辅酶A和乙酰辅酶A掺入异戊二烯衍生的碳。
Bacteria that inhabit soils and the leaves of trees partially mitigate the release of the abundant volatile organic compound, isoprene (2‐methyl‐1,3‐butadiene). While the initial steps of isoprene metabolism were identified in Rhodococcus sp. AD45 two decades ago, the isoprene metabolic pathway still remains largely undefined. Limited understanding of the functions of isoG, isoJ and aldH and uncertainty in the route of isoprene‐derived carbon into central metabolism have hindered our understanding of isoprene metabolism. These previously uncharacterised iso genes are essential in Variovorax sp. WS11, determined by targeted mutagenesis. Using combined ‘omics‐based approaches, we propose the complete isoprene metabolic pathway. Isoprene is converted to propionyl‐CoA, which is assimilated by the chromosomally encoded methylmalonyl‐CoA pathway, requiring biotin and vitamin B12, with the plasmid‐encoded methylcitrate pathway potentially providing robustness against limitations in these vitamins. Key components of this pathway were induced by both isoprene and its initial oxidation product, epoxyisoprene, the principal inducer of isoprene metabolism in both Variovorax sp. WS11 and Rhodococcus sp. AD45. Analysis of the genomes of distinct isoprene‐degrading bacteria indicated that all of the genetic components of the methylcitrate and methylmalonyl‐CoA pathways are not always present in isoprene degraders, although incorporation of isoprene‐derived carbon via propionyl‐CoA and acetyl‐CoA is universally indicated.
登录
查看更多内容
影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
DOI：
10.1073/pnas.1812668115
发表时间：
2018-12-18
影响因子：
11.1
作者：
Crombie AT;Larke-Mejia NL;Emery H;Dawson R;Pratscher J;Murphy GP;McGenity TJ;Murrell JC
通讯作者：
Murrell JC
影响因子：
3.2
作者：
FUCHS, J
通讯作者：
FUCHS, J
影响因子：
2.9
作者：
Horswill, AR;Escalante-Semerena, JC
通讯作者：
Escalante-Semerena, JC
影响因子：
2.9
作者：
Khan, M. Anwar H.;Schlich, Billie-Louise;Shallcross, Dudley E.
通讯作者：
Shallcross, Dudley E.