Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.
Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.
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DOI：
10.1038/s41467-021-26933-1
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发表时间：
2021-12-07
影响因子：
16.6
通讯作者：
Soh HT
中科院分区：
文献类型：
--
作者：
Yoshikawa AM;Rangel A;Feagin T;Chun EM;Wan L;Li A;Moeckl L;Wu D;Eisenstein M;Pitteri S;Soh HT
糖基化是翻译后修饰的最丰富形式之一，并且可以对广泛的生物过程和疾病产生深远的影响。不幸的是，表征这种修饰的生物学功能的努力由于缺乏能够以强亲和力和特异性区分蛋白糖型的亲和试剂而受到极大阻碍。在这项工作中，我们使用基于荧光激活细胞分选（FACS）的方法来产生和筛选具有吲哚修饰碱基的适体，这些适体能够识别和区分特定的蛋白质糖型。使用这种方法，我们能够选择对两种不同蛋白质的特定糖型表现出强选择性的碱基修饰的适体。这些适体可以区分仅在其聚糖修饰上不同的分子，并且还可以用于标记培养细胞表面上的糖蛋白。我们相信我们的策略应该为糖生物学研究开发有用的试剂提供一种普遍适用的方法。糖基化是翻译后修饰的一种丰富形式。在这里，作者提出了一个可推广的工作流程，用于选择吲哚修饰的适体，可以识别具有高特异性的蛋白质糖型。
Glycosylation is one of the most abundant forms of post-translational modification, and can have a profound impact on a wide range of biological processes and diseases. Unfortunately, efforts to characterize the biological function of such modifications have been greatly hampered by the lack of affinity reagents that can differentiate protein glycoforms with robust affinity and specificity. In this work, we use a fluorescence-activated cell sorting (FACS)-based approach to generate and screen aptamers with indole-modified bases, which are capable of recognizing and differentiating between specific protein glycoforms. Using this approach, we were able to select base-modified aptamers that exhibit strong selectivity for specific glycoforms of two different proteins. These aptamers can discriminate between molecules that differ only in their glycan modifications, and can also be used to label glycoproteins on the surface of cultured cells. We believe our strategy should offer a generally-applicable approach for developing useful reagents for glycobiology research. Glycosylation is an abundant form of post-translational modification. Here the authors present a generalizable workflow for the selection of indole-modified aptamers that can recognize protein glycoforms with high specificity.
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DOI：
10.1038/mtna.2014.49
发表时间：
2014-10-07
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
--
影响因子：
15
作者：
Hudson KL;Bartlett GJ;Diehl RC;Agirre J;Gallagher T;Kiessling LL;Woolfson DN
通讯作者：
Woolfson DN
影响因子：
3.6
作者：
LORAND, JP;EDWARDS, JO
通讯作者：
EDWARDS, JO
DOI：
10.3390/molecules21121613
发表时间：
2016-11-25
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Vorobyeva M;Vorobjev P;Venyaminova A
通讯作者：
Venyaminova A
影响因子：
16.6
作者：
Wang, Jinpeng;Gong, Qiang;Maheshwari, Nupur;Eisenstein, Michael;Arcila, Mary Luz;Kosik, Kenneth S.;Soh, H. Tom
通讯作者：
Soh, H. Tom