Cell paintballing using optically targeted coacervate microdroplets.
Cell paintballing using optically targeted coacervate microdroplets.
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DOI：
10.1039/c5sc02266e
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发表时间：
2015-11-01
期刊：
影响因子：
8.4
通讯作者：
Perriman AW
中科院分区：
文献类型：
--
作者：
Armstrong JPK;Olof SN;Jakimowicz MD;Hollander AP;Mann S;Davis SA;Miles MJ;Patil AJ;Perriman AW
一个动态的全息组装器被用来“彩弹”干细胞与凝聚微滴加载蛋白质，寡核苷酸或分子染料。我们提出了一种新的方法，用于以高空间精度将生物分子有效载荷定向递送到单个细胞。这是通过将蛋白质、寡核苷酸或分子染料主动螯合到凝聚微滴中来实现的，然后使用动态全息组装器将其递送到干细胞膜的特定区域，导致自发凝聚微滴-膜融合。微滴的容易制备、高隔离效率和固有的膜亲和力使得这种新型的“细胞彩弹”技术成为空间定向细胞功能化的非常有利的选择，在单细胞刺激、转染和分化中具有潜在的应用。
A dynamic holographic assembler was used to “paintball” stem cells with coacervate microdroplets loaded with protein, oligonucleotide or molecular dye. We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet–membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel “cell paintballing” technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.
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